FOSB ELISA 试剂盒
人 FOSB ELISA Kit, Colorimetric is an assay for quantification of 人 FOSB.
产品编号 ABIN4882920
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for FOSB ELISA 试剂盒 (ABIN4882920)
抗原
See all FOSB ELISA试剂盒
FOSB
(FBJ Murine Osteosarcoma Viral Oncogene Homolog B (FOSB))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.09 ng/mL - 20 ng/mL
应用范围
ELISA
样品类型
Cell Culture Supernatant, Plasma, Serum
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最低检测浓度
- 0.09 ng/mL
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原理
- Human FosB/G0S3 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
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Analytical Method
- Quantitative
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特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTSL-1/Punctin-1, AMSH/STAMBP, Annexin V, BATF3, Bora, Cadherin-17, Caveolin-2, CD2, CD200 R1, CHST3, COMT, Cystatin SA, Dopamine beta-Hydroxylase, Desmin, Exostosin-like 3, Ficolin-1, FRS2, GATA-5, GFAP, GLI-3, HepaCAM, ARNT/HIF-1 beta, 17 betaHSD1/HSD17B1, IDO, Kallikrein 1, Kell, MDL-1/CLEC5A, NPDC-1, Numb, Olig2, p63/TP73L, Pax3, Semaphorin 4D, Sphingosine Kinase 1/SPHK1, TAZ/WWTR1, TC-PTP, Transglutaminase 3/TGM3, TPST2, TREML1/TLT-1.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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FBJ Murine Osteosarcoma Viral Oncogene Homolog B (FOSB) ELISA Kit
FOSB 适用: 人 Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
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应用备注
- Suggested Plasma Dilution: 5 fold
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- FOSB (FBJ Murine Osteosarcoma Viral Oncogene Homolog B (FOSB))
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别名
- FosB
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背景
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Gene Symbols: FOSB, G0S3
Protein Name (Synonyms): Protein fosB (G0/G1 switch regulatory protein 3)
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基因ID
- 2354
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UniProt
- P53539
抗原 See all FOSB ELISA试剂盒
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