ENPP7 ELISA 试剂盒
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北京 101111
Quick Overview for ENPP7 ELISA 试剂盒 (ABIN4882722)
抗原
See all ENPP7 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 22.5 pg/mL
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原理
- Human ENPP-7/Alk-Smase ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
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Analytical Method
- Quantitative
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特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: human ANGPTL7, CD36, CLEC9a, CL-P1, Dectin-2, DLL4, DSCAM, EDIL3, Enteropeptidase, FCRL3, FCRLB, FGF-3, FLRT1, FLRT2, GBA3, GDF-11, Glycoprotein V, Granzyme A, IGSF4B, IL-28 R alpha, Kynureninase, LAMA4, LRRC4, LRRTM3, NG2, NQO-1, PCSK2, PILR-alpha, Plexin A4, POGLUT1, PRELP, Smad4, SOX15, SOX7, SOX9, Syntaxin 6, TROP-2, TSLP R, UNC5H4.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Alkaline Sphingomyelinase (Alk-Smase) ELISA Kit
适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
Alkaline Sphingomyelinase (Alk-Smase) ELISA Kit适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Alkaline Sphingomyelinase (Alk-Smase) ELISA Kit适用: 人 AA 22-439 Colorimetric Sandwich ELISA 93.7 pg/mL - 6000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Alkaline Sphingomyelinase (Alk-Smase) ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
Alkaline Sphingomyelinase (Alk-Smase) ELISA Kit适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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应用备注
- Suggested Plasma Dilution: 10 fold
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- ENPP7 (Alkaline Sphingomyelinase (Alk-Smase))
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别名
- ENPP-7
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背景
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Gene Symbols: ENPP7, UNQ3077/PRO9912
Protein Name (Synonyms): Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 (E-NPP 7) (NPP-7) (EC 3.1.4.12) (Alkaline sphingomyelin phosphodiesterase) (Intestinal alkaline sphingomyelinase) (Alk-SMase)
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基因ID
- 339221
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UniProt
- Q6UWV6
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