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EGR1 ELISA 试剂盒

EGR1 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN4882701
发货至: 中国
  • 抗原 See all EGR1 ELISA试剂盒
    EGR1 (Early Growth Response 1 (EGR1))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human EGR1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human EGR1.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all EGR1 ELISA试剂盒
    EGR1 (Early Growth Response 1 (EGR1))
    别名
    EGR1 (EGR1 产品)
    别名
    AT225 ELISA Kit, G0S30 ELISA Kit, KROX-24 ELISA Kit, NGFI-A ELISA Kit, TIS8 ELISA Kit, ZIF-268 ELISA Kit, ZNF225 ELISA Kit, A530045N19Rik ELISA Kit, ETR103 ELISA Kit, Egr-1 ELISA Kit, Krox-1 ELISA Kit, Krox-24 ELISA Kit, Krox24 ELISA Kit, NGF1-A ELISA Kit, NGFIA ELISA Kit, Zenk ELISA Kit, Zfp-6 ELISA Kit, Zif268 ELISA Kit, egr ELISA Kit, Ngf1 ELISA Kit, Ngfi ELISA Kit, zif-268 ELISA Kit, EGR1 ELISA Kit, EGR-1-A ELISA Kit, Xegr-1 ELISA Kit, at225 ELISA Kit, egr-1 ELISA Kit, egr1 ELISA Kit, g0s30 ELISA Kit, krox-24 ELISA Kit, ngfi-a ELISA Kit, tis8 ELISA Kit, znf225 ELISA Kit, krox24 ELISA Kit, wu:fj64b05 ELISA Kit, wu:fq25f01 ELISA Kit, EGR-1 ELISA Kit, zenk ELISA Kit, EGR-1-B ELISA Kit, egr1-a ELISA Kit, egr1-b ELISA Kit, early growth response 1 ELISA Kit, early growth response protein 1 (egr-1) ELISA Kit, early growth response 1 L homeolog ELISA Kit, early growth response 1 S homeolog ELISA Kit, EGR1 ELISA Kit, CNK00890 ELISA Kit, Egr1 ELISA Kit, egr1.L ELISA Kit, egr1 ELISA Kit, egr1.S ELISA Kit
    基因ID
    1958
    UniProt
    P18146
    途径
    Regulation of Carbohydrate Metabolic Process, Regulation of long-term Neuronal Synaptic Plasticity
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