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Factor XI ELISA 试剂盒

This anti-Factor XI antibody (ABIN4882429) is a antibody detecting Factor XI in ELISA. Suitable for Human.
产品编号 ABIN4882429
发货至: 中国

Quick Overview for Factor XI ELISA 试剂盒 (ABIN4882429)

抗原

See all Factor XI (F11) ELISA试剂盒
Factor XI (F11) (Coagulation Factor XI (F11))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

应用范围

ELISA

样品类型

Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
  • 原理

    Custom Human Coagulation Factor 11 (F11) ELISA Kit.

    Analytical Method

    Quantitative

    特异性

    The antibody pair provided in this kit recognizes Human Coagulation Factor 11 (F11)

    产品特性

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    组件

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    试剂未包括

    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • 样本量

    100 μL

    板类型

    Pre-coated

    实验流程

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    限制

    仅限研究用
  • 储存条件

    -20 °C

    储存方法

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    有效期

    6 months
  • 抗原 See all Factor XI (F11) ELISA试剂盒

    Factor XI (F11) (Coagulation Factor XI (F11))

    别名

    Coagulation Factor XI

    基因ID

    2160

    UniProt

    P03951
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