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CES1 ELISA 试剂盒

CES1 适用: 人 Colorimetric Sandwich ELISA 28.67-7000 pg/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN4882305
发货至: 中国
  • 抗原 See all CES1 ELISA试剂盒
    CES1 (Carboxylesterase 1 (CES1))
    适用
    • 3
    • 2
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    28.67-7000 pg/mL
    最低检测浓度
    28.67 pg/mL
    应用范围
    ELISA
    原理
    Human Carboxylesterase 1/CES1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human Carboxylesterase 1.
    灵敏度
    28 pg/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 应用备注
    Recommended Dilution for serum and plasma samples50 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all CES1 ELISA试剂盒
    CES1 (Carboxylesterase 1 (CES1))
    别名
    Carboxylesterase 1 (CES1 产品)
    别名
    ACAT ELISA Kit, CE-1 ELISA Kit, CEH ELISA Kit, CES2 ELISA Kit, HMSE ELISA Kit, HMSE1 ELISA Kit, PCE-1 ELISA Kit, REH ELISA Kit, SES1 ELISA Kit, TGH ELISA Kit, hCE-1 ELISA Kit, CESDD1 ELISA Kit, CES1 ELISA Kit, CES-K1 ELISA Kit, APLE ELISA Kit, PMPMEase ELISA Kit, CES ELISA Kit, carboxylesterase 1 ELISA Kit, liver carboxylesterase 1 ELISA Kit, liver carboxylesterase ELISA Kit, carboxylesterase 1 (monocyte/macrophage serine esterase 1) ELISA Kit, CES1 ELISA Kit, CpipJ_CPIJ013026 ELISA Kit, CpipJ_CPIJ016339 ELISA Kit, LOC100009551 ELISA Kit, LOC454097 ELISA Kit, LOC699486 ELISA Kit, LOC100050915 ELISA Kit
    基因ID
    1066
    UniProt
    P23141
    途径
    Monocarboxylic Acid Catabolic Process
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