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CD147 ELISA 试剂盒

BSG 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
产品编号 ABIN4882135
发货至: 中国
  • 抗原 See all CD147 (BSG) ELISA试剂盒
    CD147 (BSG) (Basigin (Ok Blood Group) (BSG))
    适用
    • 8
    • 5
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Custom Human EMMPRIN (CD147) ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    The antibody pair provided in this kit recognizes Human EMMPRIN (CD147)
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all CD147 (BSG) ELISA试剂盒
    CD147 (BSG) (Basigin (Ok Blood Group) (BSG))
    别名
    CD147 (BSG 产品)
    别名
    EMMPRIN ELISA Kit, RPE7 ELISA Kit, 5F7 ELISA Kit, CD147 ELISA Kit, M6 ELISA Kit, OK ELISA Kit, TCSF ELISA Kit, AI115436 ELISA Kit, AI325119 ELISA Kit, HT-7 ELISA Kit, 5A11 ELISA Kit, CE9 ELISA Kit, HT7 ELISA Kit, Ox47R ELISA Kit, basignin ELISA Kit, wu:fb26a05 ELISA Kit, wu:fo98e12 ELISA Kit, bsg ELISA Kit, prso03 ELISA Kit, basigin (Ok blood group) ELISA Kit, basigin ELISA Kit, basigin (Ok blood group) S homeolog ELISA Kit, BSG ELISA Kit, Bsg ELISA Kit, bsg ELISA Kit, bsg.S ELISA Kit
    基因ID
    682
    UniProt
    P35613
    途径
    S100 Proteins
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