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BCAN ELISA 试剂盒

BCAN 适用: 人 Colorimetric Sandwich ELISA 0.041-10 ng/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN4881943
发货至: 中国
  • 抗原 See all BCAN ELISA试剂盒
    BCAN (Brevican (BCAN))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.041-10 ng/mL
    最低检测浓度
    0.041 ng/mL
    应用范围
    ELISA
    原理
    Human Brevican ELISA Kit for serum, plasma, and cell culture supernatant samples.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human 4-1BB Ligand, Activin RIIB, Aminopeptidase P2, BAMBI, BOC, Carbonic Anhydrase XII, Carboxypeptidase A2, CD300c (LMIR2), CD320 (TCblR, 8D6A), CDNF, CDO, CHST1, CHST4, CILP-1, CNTF R alpha, CRIM1, CRTAC1, CXADR, Dopa Decarboxylase, DPPII, DSPG3, EMR2, FCAR (CD89), FCRL1 (FcRH1), FCRL2 (FcRH2), Gas6, GPR56, GPVI, Hepsin, ILT2 (CD85j), Jagged 2, KLF4, LAIR1, LAMP, LAMP1 (CD107a), MDGA1, MIS RII, Neurexin 3.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 应用备注
    Recommended Dilution for serum and plasma samples2 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all BCAN ELISA试剂盒
    BCAN (Brevican (BCAN))
    别名
    Brevican (BCAN 产品)
    别名
    fi32a12 ELISA Kit, wu:fi32a12 ELISA Kit, BCAN ELISA Kit, Xbcan ELISA Kit, Cspg7 ELISA Kit, ALPBRE ELISA Kit, BEHAB ELISA Kit, CSPG7 ELISA Kit, brevican ELISA Kit, brevican L homeolog ELISA Kit, bcan ELISA Kit, BCAN ELISA Kit, bcan.L ELISA Kit, Bcan ELISA Kit
    基因ID
    63827
    UniProt
    Q96GW7
    途径
    Glycosaminoglycan Metabolic Process
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