AHCY ELISA 试剂盒
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Quick Overview for AHCY ELISA 试剂盒 (ABIN4881774)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.61 ng/mL
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原理
- Human AHCY ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
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Analytical Method
- Quantitative
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特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: human Activin C, APE, Bax, BNIP3, Caspase-3, Cathepsin F, CD177, CD96, CLEC4D, C-Peptide, DCBLD2, Dystroglycan, FAM3A, GCNT1, GLA, HAPLN4, hCG alpha, HMGB3, IkB-E, IRF4, KIR3DL1, Klotho beta, Lck, LILRA5, METTL11A, p55PIK, PCDH17, PLA2G4A, RalB, S100A2, S100B, SH2B1, Siglec-3, SOD3, ST8SIA2, Testican 1, TRP14, VAP-B, ZAP70.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Adenosylhomocysteinase (AHCY) ELISA Kit
AHCY 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Adenosylhomocysteinase (AHCY) ELISA KitAHCY 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Adenosylhomocysteinase (AHCY) ELISA KitAHCY 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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应用备注
- Suggested Plasma Dilution: 2 fold
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- AHCY (Adenosylhomocysteinase (AHCY))
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别名
- AHCY
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背景
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Gene Symbols: AHCY, SAHH
Protein Name (Synonyms): Adenosylhomocysteinase (AdoHcyase) (EC 3.3.1.1) (S-adenosyl-L-homocysteine hydrolase)
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基因ID
- 191
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UniProt
- P23526
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