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GLA ELISA 试剂盒

GLA 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
产品编号 ABIN4881769
发货至: 中国
  • 抗原 See all GLA ELISA试剂盒
    GLA (Galactosidase, alpha (GLA))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Custom Human alpha-Galactosidase A/GLA ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes .
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all GLA ELISA试剂盒
    GLA (Galactosidase, alpha (GLA))
    别名
    alpha-Galactosidase A (GLA 产品)
    别名
    GALA ELISA Kit, Ags ELISA Kit, zgc:101584 ELISA Kit, MGC130872 ELISA Kit, SMU.877 ELISA Kit, SCF11.21 ELISA Kit, AO090005000217 ELISA Kit, alpha-GAL ELISA Kit, galactosidase alpha ELISA Kit, galactosidase, alpha ELISA Kit, galactosidase alpha S homeolog ELISA Kit, alpha-galactosidase ELISA Kit, aga ELISA Kit, alpha-galactosidase A ELISA Kit, GLA ELISA Kit, Gla ELISA Kit, gla ELISA Kit, gla.S ELISA Kit, agaN ELISA Kit, aga ELISA Kit, agaL ELISA Kit, SCO0541 ELISA Kit, rafA ELISA Kit, melA ELISA Kit, galA ELISA Kit, ANI_1_2528074 ELISA Kit, ANI_1_1502124 ELISA Kit, AOR_1_390174 ELISA Kit, CpipJ_CPIJ002066 ELISA Kit, MCYG_00962 ELISA Kit, MCYG_00791 ELISA Kit, Tsp_02909 ELISA Kit, Tsp_02508 ELISA Kit
    基因ID
    2717
    UniProt
    P06280
    途径
    SARS-CoV-2 Protein Interactome
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