Substance P ELISA 试剂盒
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北京 101111
Quick Overview for Substance P ELISA 试剂盒 (ABIN4881333)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.1 ng/mL
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原理
- Human/Mouse/Rat Substance P EIA Kit optimized for serum, plasma, and cell culture supernatants. Competition-based ELISA
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Analytical Method
- Quantitative
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特异性
- This EIA kit is designed to detect human, mouse, and rat Substance P.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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Substance P ELISA Kit
适用: 大鼠 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Substance P ELISA Kit适用: 大鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Substance P ELISA Kit适用: 犬 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Substance P ELISA Kit适用: 豚鼠 Colorimetric Sandwich ELISA 47 pg/mL - 3000 pg/mL Plasma, Serum
Substance P ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Substance P ELISA Kit适用: Pig Colorimetric Sandwich ELISA 0.25 ng/mL - 16 ng/mL Plasma, Serum, Tissue Homogenate
Substance P ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Substance P ELISA Kit适用: 大鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Substance P ELISA Kit适用: 人 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Substance P ELISA Kit适用: 人 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Suggested Plasma Dilution: Human: 2x / Mouse: 8x / Rat: 8X
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4 °C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
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有效期
- 6 months
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- Substance P
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背景
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Gene Symbols: TAC1, NKA, NKNA, TAC2
Background: Substance P (SP) is an 11 amino acid peptide produced from a precursor protein, Protachykinin-1. It is a neuropeptide, acting as a neurotransmitter and as a neuromodulator. It is found in the brain and spinal cord and is associated with inflammatory processes and pain. Its receptor, NK1R, is distributed over cytoplasmic and nuclear membranes of many cell types (neurons, glia, endothelia of capillaries and lymphatics, fibroblasts, stem cells, white blood cells) in many tissues and organs. Substance P has been found as a key first responder to most noxious/extreme stimuli (stressors). It has been reported that Substance P is involved in many biological events, such as vasodilation, inflammation, pain, mood, anxiety, Learning, vomiting, cell growth, proliferation, angiogenesis, and migration. Elevation of Substance P and/or its receptor (NK1R) in serum, plasma, or tissue has been associated with many diseases.
Protein Name (Synonyms): Protachykinin-1 (PPT) [Cleaved into: Substance P Neurokinin A (NKA) (Neuromedin L) (Substance K) Neuropeptide K (NPK) Neuropeptide gamma C-terminal-flanking peptide]
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基因ID
- 6863
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UniProt
- P20366
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