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Neurotensin ELISA 试剂盒

NTS 适用: 人, 大鼠, 小鼠 Colorimetric Competition ELISA 0.1-1.000 ng/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN4881315
发货至: 中国
  • 抗原 See all Neurotensin (NTS) ELISA试剂盒
    Neurotensin (NTS)
    适用
    • 6
    • 4
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    • 2
    • 2
    • 1
    • 1
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    • 1
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    • 1
    人, 大鼠, 小鼠
    检测方法
    Colorimetric
    实验类型
    Competition ELISA
    检测范围
    0.1-1.000 ng/mL
    最低检测浓度
    0.1 ng/mL
    应用范围
    ELISA
    原理
    Human/Mouse/Rat Neurotensin EIA Kit optimized for serum, plasma and cell culture supernatants. Competition-based ELISA on a 96-well strip plate.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    This EIA kit is designed to detect human, mouse, and rat Neurotensin .
    灵敏度
    0.2 ng/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Standard Peptide
    • Assay Diluent(s)
    • Biotinylated Peptide
    • HRP-Streptavidin
    • TMB One-Step Substrate
    • Stop Solution
    • Assay Diagram
    • Positive Control Sample
    • Capture Antibody
    • User Manual
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Orbital shaker
    • Aluminum foil
    • Saran Wrap
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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  • 应用备注
    Recommended Dilution for serum and plasma samplesHuman: 2X / Mouse: 8X / Rat: 8X
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed.
    2. Add 100 μL detection antibody to each well.
    3. Incubate 1.5 h at RT or O/N at 4 °C.
    4. Add 100 μL standard or sample to each well.
    5. Incubate 2.5 h at RT.
    6. Add 100 μL prepared streptavidin solution.
    7. Incubate 45 min at RT.
    8. Add 100 μL TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL Stop Solution to each well.
    11. Read plate at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    Standard, Biotinylated peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
    有效期
    6 months
  • 抗原 See all Neurotensin (NTS) ELISA试剂盒
    Neurotensin (NTS)
    别名
    Neurotensin (NTS 产品)
    别名
    NTS ELISA Kit, NMN-125 ELISA Kit, NN ELISA Kit, NT ELISA Kit, NT/N ELISA Kit, NTS1 ELISA Kit, 5033428E16Rik ELISA Kit, neurotensin ELISA Kit, NTS ELISA Kit, Nts ELISA Kit
    基因ID
    4922
    UniProt
    P30990
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