Neurotensin ELISA 试剂盒
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Quick Overview for Neurotensin ELISA 试剂盒 (ABIN4881315)
抗原
See all Neurotensin (NTS) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.1 ng/mL
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原理
- Human/Mouse/Rat Neurotensin EIA Kit optimized for serum, plasma and cell culture supernatants. Competition-based ELISA
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Analytical Method
- Quantitative
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特异性
- This EIA kit is designed to detect human, mouse, and rat Neurotensin .
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灵敏度
- 0.2 ng/mL
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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Neurotensin (NTS) ELISA Kit
NTS 适用: 人 Colorimetric Competition ELISA 37.0 pg/mL - 3000 pg/mL Plasma, Serum
Neurotensin (NTS) ELISA KitNTS 适用: 大鼠 Colorimetric Competition ELISA 37.0 pg/mL - 3000 pg/mL Plasma, Serum
Neurotensin (NTS) ELISA KitNTS 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neurotensin (NTS) ELISA KitNTS 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
Neurotensin (NTS) ELISA KitNTS 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Neurotensin (NTS) ELISA KitNTS 适用: 人 Colorimetric Competition ELISA 1.56 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
Neurotensin (NTS) ELISA KitNTS 适用: 小鼠 Colorimetric Competition ELISA 1.56 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
Neurotensin (NTS) ELISA KitNTS 适用: 大鼠 Colorimetric Competition ELISA 1.56 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
Neurotensin (NTS) ELISA KitHigh Sensitivity NTS 适用: 人 Colorimetric Competition ELISA 3.7 pg/mL - 300 pg/mL Plasma, Serum
Neurotensin (NTS) ELISA KitNTS 适用: 人 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Suggested Plasma Dilution: Human: 2X / Mouse: 8X / Rat: 8X
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4 °C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- Standard, Biotinylated Neurotensin peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
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有效期
- 6 months
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- Neurotensin (NTS)
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别名
- Neurotensin
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背景
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Gene Symbols: NTS
Background: Neurotensin is a 13 amino acid neuropeptide that is widely distributed throughoutthe nervous system, and may function as a neurotransmitter or a neuromodulator. Itacts on three subtypes of neurotensin receptors which have many central andperipheral actions.
Neurotensin induces a variety of effects, including: analgesia, hypothermia andincreased locomotor activity. It may be involved in dopamine-associatedpathophysiological events.
In the periphery, Neurotensin is found in enteroendocrine cells of the smallintestine. It may be involved in the maintenance of gut structure and function, and inthe regulation of fat metabolism. It is a potent mitogen for colorectal cancer.Neurotensin also exhibits antimicrobial activity against bacteria and fungi.Protein Name (Synonyms): Neurotensin/neuromedin N [Cleaved into: Large neuromedin N (NmN-125) Neuromedin N (NN) (NmN) Neurotensin (NT) Tail peptide]
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基因ID
- 4922
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UniProt
- P30990
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