NUCB2 ELISA 试剂盒
(2 references)Quick Overview for NUCB2 ELISA 试剂盒 (ABIN4881312)
抗原
See all NUCB2 (NES1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.1 ng/mL
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原理
- Human/Mouse/Rat Nesfatin EIA Kit optimized for serum and cell culture supernatants. Competition-based ELISA on a 96-well strip plate.
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Analytical Method
- Quantitative
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特异性
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This kit detects human, mouse and rat 82aa Nesfatin protein. No other active isoforms have been reported.
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灵敏度
- 400 pg/mL
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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Nesfatin-1 (NES1) ELISA Kit
NES1 适用: 人 Colorimetric Competition ELISA 617.3 pg/mL - 50000 pg/mL Plasma, Serum
Nesfatin-1 (NES1) ELISA KitNES1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Nesfatin-1 (NES1) ELISA KitNES1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Nesfatin-1 (NES1) ELISA KitNES1 适用: 大鼠 Colorimetric Competition ELISA 493.8 pg/mL - 40000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nesfatin-1 (NES1) ELISA KitNES1 适用: 人 Colorimetric Competition ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nesfatin-1 (NES1) ELISA KitNES1 适用: 人 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Nesfatin-1 (NES1) ELISA KitNES1 适用: 小鼠 Colorimetric Competition ELISA 493.8 pg/mL - 40000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nesfatin-1 (NES1) ELISA KitSmall Sample NES1 适用: 小鼠 Colorimetric Competition ELISA 493.8 pg/mL - 40000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Recommended Dilution for serum and plasma samplesHuman: 2X / Mouse: 2X / Rat: 2X
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4 °C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
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有效期
- 6 months
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: "The evaluation of Nesfatin-1 levels in patients with and without intrauterine growth restriction." in: The journal of maternal-fetal & neonatal medicine, Vol. 29, Issue 9, pp. 1409-13, (2016) (PubMed).
: "The relation of serum nesfatin-1 level with metabolic and clinical parameters in obese and healthy children." in: Pediatric diabetes, Vol. 14, Issue 3, pp. 189-95, (2013) (PubMed).
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: "The evaluation of Nesfatin-1 levels in patients with and without intrauterine growth restriction." in: The journal of maternal-fetal & neonatal medicine, Vol. 29, Issue 9, pp. 1409-13, (2016) (PubMed).
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- NUCB2 (NES1) (Nesfatin-1 (NES1))
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别名
- Nesfatin
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基因ID
- 4925
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UniProt
- P80303
抗原 See all NUCB2 (NES1) ELISA试剂盒
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