Chromogranin A ELISA 试剂盒
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北京 101111
Quick Overview for Chromogranin A ELISA 试剂盒 (ABIN4881303)
抗原
See all Chromogranin A (CHGA) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.1 ng/mL
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原理
- Human/Mouse/Rat Catestatin EIA Kit optimized for serum, plasma and cell culture supernatants. Competition-based ELISA
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Analytical Method
- Quantitative
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特异性
- This EIA kit is designed to detect human, mouse, and rat Catestatin.
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灵敏度
- 0.5 ng/mL
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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Chromogranin A (CHGA) ELISA Kit
CHGA 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Chromogranin A (CHGA) ELISA KitCHGA 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum, Urine
Chromogranin A (CHGA) ELISA KitCHGA 适用: 人 Colorimetric Sandwich ELISA 62.5 ng/mL - 4000 ng/mL Cell Culture Supernatant, Cerebrospinal Fluid, Plasma, Serum, Urine
Chromogranin A (CHGA) ELISA KitCHGA 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chromogranin A (CHGA) ELISA KitCHGA 适用: 人 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
Chromogranin A (CHGA) ELISA KitCHGA 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Chromogranin A (CHGA) ELISA KitCHGA 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Chromogranin A (CHGA) ELISA KitCHGA 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chromogranin A (CHGA) ELISA KitCHGA 适用: 小鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Chromogranin A (CHGA) ELISA KitCHGA 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
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应用备注
- Suggested Plasma Dilution: Human: 12X / Mouse: 12X / Rat: 8X
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4 °C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- Standard, Biotinylated Catestatin peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
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有效期
- 6 months
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- Chromogranin A (CHGA)
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别名
- Catestatin
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背景
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Gene Symbols: CHGA
Background: Catestatin is a 21-amino acid peptide that is cleaved from its precursor chromogranin A. As a potent nicotinic cholinergic antagonist, Catestatin inhibits catecholamine release from chromaffin cells and noradrenergic neurons. It also can induce mast cell migration, degranulation and stimulate histamine release. It has been suggested that early deficiency of Catestatin might play a pathogenic role in the subsequent development of the hypertension.
Catestatin also acts on innate immunity, and shows antibacterial activity against Gram-positive and Gram-negative bacteria.Protein Name (Synonyms): Chromogranin-A (CgA) (Pituitary secretory protein I) (SP-I) [Cleaved into: Vasostatin-1 (Vasostatin I) Vasostatin-2 (Vasostatin II) EA-92 ES-43 Pancreastatin SS-18 WA-8 WE-14 LF-19 AL-11 GV-19 GR-44 ER-37]
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基因ID
- 1113
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UniProt
- P10645
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途径
- Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling
抗原 See all Chromogranin A (CHGA) ELISA试剂盒
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