CAMP ELISA 试剂盒
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- 抗原 See all CAMP (cAMP) ELISA试剂盒
- CAMP (cAMP) (Cyclic Adenosine Monophosphate (cAMP))
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适用
- 化学剂
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 0.1-1.000 ng/mL
- 最低检测浓度
- 0.1 ng/mL
- 应用范围
- ELISA
- 原理
- cAMP EIA Kit optimized for serum and plasma samples. Competition-based ELISA on a 96-well strip plate.
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This EIA kit is designed to only detect Cyclic AMP.
- 灵敏度
- 4.4 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
- Top Product
- Discover our top product cAMP ELISA Kit
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Cyclic Adenosine Monophosphate (cAMP) ELISA Kit
cAMP 适用: Various Species Colorimetric Competition ELISA 246.9 pg/mL - 20000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cyclic Adenosine Monophosphate (cAMP) ELISA KitcAMP 适用: Various Species Colorimetric Sandwich ELISA Cell Lysate, Plasma (EDTA), Plasma (heparin), Saliva, Tissue Culture Medium, Urine
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4 °C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Standard, Biotinylated cAMP peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
- 有效期
- 6 months
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- 抗原 See all CAMP (cAMP) ELISA试剂盒
- CAMP (cAMP) (Cyclic Adenosine Monophosphate (cAMP))
- 别名
- cAMP, Cyclic AMP (cAMP 产品)
- 别名
- CRAMP ELISA Kit, CAP-18 ELISA Kit, CAP18 ELISA Kit, FALL-39 ELISA Kit, FALL39 ELISA Kit, LL37 ELISA Kit, CAP11 ELISA Kit, Cnlp ELISA Kit, Cramp ELISA Kit, MCLP ELISA Kit, CATHL7 ELISA Kit, cathelicidin antimicrobial peptide ELISA Kit, Camp ELISA Kit, CAMP ELISA Kit
- 物质类
- Chemical
- 途径
- Cellular Response to Molecule of Bacterial Origin
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