EBV VCA IgM ELISA 试剂盒
This 人 EBV VCA IgM ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 EBV VCA IgM.
Quick Overview for EBV VCA IgM ELISA 试剂盒 (ABIN459379)
抗原
See all EBV VCA IgM products
EBV VCA IgM
(Anti-Epstein-Barr Virus Viral Capsid Antigen IgM (EBV VCA IgM))
适用
人
检测方法
Colorimetric
实验类型
Indirect ELISA
应用范围
ELISA
样品类型
Serum
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原理
- ELISA-VIDITEST anti-VCA EBV IgM assay is a solid-phase immunoanalytical test.
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品牌
- ELISA-VIDITEST
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Analytical Method
- Semi-Quantitative
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组件
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- ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
- 1.3 mL Negative control human serum, r.t.u. 1) NC 1 vial
- 1.3 mL Positive control human serum, r.t.u. PC 1 vial
- 2.0 mL Calibrator (human serum), r.t.u. CAL 1 vial
- 13 mL Anti-human IgM animal antibodies labelled with horseradish peroxidase (anti-IgM Px conjugate) r.t.u. CONJ-V 1 vial
- 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
- 60 mL Dilution buffer, r.t.u. DIL 1 vial
- 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H2O2) TMB 1 vial
- 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
- 2 mL RF-sorbent (anti-human IgG) 25 x concentrated RF SORB 25x 1 vial
- Instruction manual
- Quality Control Certificate
- 1) r.t.u., ready to use
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试剂未包括
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- Distilled or deionised water for dilution of buffer and standard concentrates
- appropriate and calibrated equipment for pipetting
- temperature controlled incubator
- spectrophotometer or platereader with the appropriate filters
- empty microtiter plate for pre-incubation
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
- anti-VCA EBV IgM assay is a solid-phase immunoanalytical test. The strips are coated with specific antigen that bears immunodominant epitopes of VCA complex. If relevant antibodies are present in the test samples, they bind to the immobilized antigen. The bound antibodies then in a next step react with horseradish peroxidase-labeled anti-human IgM antibodies. The amount of labeled antibodies bound is determined by the color enzymatic reaction. Negative samples do not react, a mild change in the colour of the wells is the reaction background.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- EBV VCA IgM (Anti-Epstein-Barr Virus Viral Capsid Antigen IgM (EBV VCA IgM))
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物质类
- Antibody
抗原 See all EBV VCA IgM products
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