Epstein-Barr Virus VCA IgG and IgG Avidity ELISA 试剂盒
The 人 Epstein-Barr Virus VCA IgG and IgG Avidity ELISA Kit (ABIN459378) is a Colorimetric ELISA Kit designed to quantify 人 Epstein-Barr Virus VCA IgG and IgG Avidity.
产品编号 ABIN459378
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Epstein-Barr Virus VCA IgG and IgG Avidity ELISA 试剂盒 (ABIN459378)
抗原
Epstein-Barr Virus VCA IgG and IgG Avidity
适用
人
检测方法
Colorimetric
实验类型
Indirect ELISA
应用范围
ELISA
样品类型
Serum, Plasma
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原理
- anti-VCA EBV IgG and IgG avidity is a solid-phase immunoanalytical test.
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品牌
- ELISA-VIDITEST
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Analytical Method
- Semi-Quantitative
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组件
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- ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
- 1.3 mL of positive control human serum containing high avidity antibodies (High avidity control serum) r.t.u.1) HIGH AVID 1 vial
- 1.3 mL of positive control human serum containing low avidity antibodies (Low avidity control serum) r.t.u. LOW AVID 1 vial
- 1.3 mL Standard A = Negative control human serum, r.t.u. ST A/NC 1 vial
- 2.0 mL Standard D = Calibrator (human serum), r.t.u. ST D/CAL 1 vial
- / 1.3 mL Standard E = Positive control human serum, r.t.u. ST E/PC 1 vial
- 13 mL Anti-human IgG animal antibodies labelled with horseradish peroxidase (anti-IgG Px conjugate) r.t.u. CONJ 1 vial
- 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
- 60 mL Dilution buffer, r.t.u. DIL 1 vial
- 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H2O2) TMB 1 vial
- 13 mL Urea solution, r.t.u. UREA 1 vial
- 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
- Instruction manual
- Quality Control Certificate
- 1) r.t.u., ready to use
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试剂未包括
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- Distilled or deionised water for dilution of buffer and standard concentrates
- appropriate and calibrated equipment for pipetting
- temperature controlled incubator
- spectrophotometer or platereader with the appropriate filters
- empty microtiter plate for pre-incubation
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
- anti-VCA EBV IgG and IgG avidity is a solid-phase immunoanalytical test. A specific antigen representing immunodominant epitopes of the VCA complex is bound to the surface of the wells. If antibodies are present in the test samples, they will bind to the immobilized proteins. The bound antibodies then react in the next step with horseradish peroxidase-labeled anti-human IgG antibodies. The amount of bound labeled antibodies is determined by a color enzymatic reaction. Negative samples do not react, a slight change in the color of the wells is the background of the reaction. To determine avidity, each sample is applied in parallel to two wells, with the appropriate antibodies binding to the immobilized antigens. In the next step, one well is incubated with the wash solution, the other well with the urea solution. In the first well, specific antibodies with high and low avidity remain bound to the antigen. In the second well, low-avidity antibodies are released due to the concentrated urea solution, and only high-avidity antibodies remain complex with the antigen. The ratio between the optical density of the well with the urea solution and the well with the washing solution in percent expresses the relative avidity index (RAI).
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限制
- 仅限研究用
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储存条件
- 4 °C
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- Epstein-Barr Virus VCA IgG and IgG Avidity
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物质类
- Antibody
抗原
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