EBV VCA IgA ELISA 试剂盒
This 人 EBV VCA IgA ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 EBV VCA IgA.
Quick Overview for EBV VCA IgA ELISA 试剂盒 (ABIN459374)
抗原
EBV VCA IgA
(Anti-Epstein-Barr Virus Viral Capsid Antigen IgA (EBV VCA IgA))
适用
人
检测方法
Colorimetric
实验类型
Indirect ELISA
应用范围
ELISA
样品类型
Serum
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原理
- anti-VCA EBV IgA is a solid-phase immunoanalytical test.
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品牌
- ELISA-VIDITEST
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Analytical Method
- Semi-Quantitative
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组件
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- ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
- 1.3 mL Negative control human serum, r.t.u. 1) NC 1 vial
- 1.3 mL Positive control human serum, r.t.u. PC 1 vial
- 2 mL Calibrator (human serum), r.t.u. CAL 1 vial
- 13 mL Anti-human IgA animal antibodies labelled with horseradish peroxidase (anti-IgA Px conjugate) r.t.u. CONJ 1 vial
- 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
- 60 mL Dilution buffer, r.t.u. DIL 1 vial
- 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H 2O2) TMB 1 vial
- 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
- Instruction manual
- Quality Control Certificate
- 1) r.t.u., ready to use
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试剂未包括
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- Distilled or deionised water for dilution of buffer and standard concentrates
- appropriate and calibrated equipment for pipetting
- temperature controlled incubator
- spectrophotometer or platereader with the appropriate filters
- empty microtiter plate for pre-incubation
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
- anti-VCA EBV IgA is a solid-phase immunoanalytical test. A specific antigen representing immunodominant epitopes of the VCA complex is bound to the surface of the wells. If antibodies are present in the test samples, they will bind to the immobilized proteins. The bound antibodies then react in the next step with horseradish peroxidase-labeled anti-human IgA antibodies. The amount of bound labeled antibodies is determined by a color enzymatic reaction. Negative samples do not react, a slight change in the color of the wells is the background of the reaction.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- EBV VCA IgA (Anti-Epstein-Barr Virus Viral Capsid Antigen IgA (EBV VCA IgA))
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物质类
- Antibody
抗原
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