TBEV IgG ELISA 试剂盒
This 人 TBEV IgG ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 TBEV IgG.
Quick Overview for TBEV IgG ELISA 试剂盒 (ABIN459371)
抗原
TBEV IgG
(Anti-Tick-Borne Encephalitis Virus Antibody IgG (TBEV IgG))
适用
人
检测方法
Colorimetric
实验类型
Indirect ELISA
应用范围
ELISA
样品类型
Cerebrospinal Fluid, Plasma, Serum
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原理
- anti-TBEV IgG (CSF) assay is a solid-phase immunoanalytical test.
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品牌
- ELISA-VIDITEST
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Analytical Method
- Semi-Quantitative
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组件
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- ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
- 1.3 mL Standard A = Negative control human serum, r.t.u. 1) ST A/NC 1 vial
- 2.0 mL Standard D = Calibrator (human serum), r.t.u. ST D/CAL 1 vial
- 1.3 mL Standard E = Positive control human serum, r.t.u. ST E/PC 1 vial
- 13 mL Anti-human IgG animal antibodies labelled with horseradish peroxidase (anti-IgG Px conjugate) r.t.u. CONJ 1 vial
- 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
- 60 mL Dilution buffer, r.t.u. DIL 1 vial
- 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H 2O2) TMB 1 vial
- 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
- / Instruction manual
- Quality Control Certificate
- 1) r.t.u., ready to use
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试剂未包括
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- Distilled or deionised water for dilution of buffer and standard concentrates
- appropriate and calibrated equipment for pipetting
- temperature controlled incubator
- spectrophotometer or platereader with the appropriate filters
- empty microtiter plate for pre-incubation
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
- anti-TBEV IgG (CSF) assay is a solid-phase immunoanalytical test. The polystyrene strips are coated with specific antigen which bear immunodominant epitopes of TBEV. Anti-TBEV antibodies in samples bind to the immobilized antigens. The antibodies that do not bind are washed away and those that formed complexes with the antigens are later on recognised by animal anti-human IgG antibodies labelled with horseradish peroxidase. The presence of labelled antibodies is revealed by an enzymatic reaction with a chromogenic substrate. Negative samples do not react and the mild change in colour, if present, may be attributed to the reaction background. Intrathecal antibody testing provides information on the anti-TBEV antibody response in the central nervous system. For this assay, it is necessary to quantify the concentration of TBEV-specific IgG antibodies in paired serum / plasma and cerebrospinal fluid samples taken from the patient at the same time. Accurate quantification of antibodies can only be performed according to the linear part of the calibration curve. Therefore, it is recommended to test the serum in two dilutions (see Chapter 6 Reagent preparation). The total IgG and albumin concentrations in both samples must be known for the determination. The calculation of intrathecal synthesis of specific antibodies is performed using the Reiber equation (see Chapter 8.4 Intrathecal synthesis of specific IgG antibodies).
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限制
- 仅限研究用
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储存条件
- 4 °C
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抗原
- TBEV IgG (Anti-Tick-Borne Encephalitis Virus Antibody IgG (TBEV IgG))
物质类
- Antibody
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