EBV EA(D) IgG ELISA 试剂盒
This 人 EBV EA(D) IgG ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 EBV EA(D) IgG.
Quick Overview for EBV EA(D) IgG ELISA 试剂盒 (ABIN459354)
抗原
See all EBV EA(D) IgG products
EBV EA(D) IgG
(Anti-Epstein-Barr Virus Early Antigen (D) IgG (EBV EA(D) IgG))
适用
人
检测方法
Colorimetric
实验类型
Indirect ELISA
应用范围
ELISA
样品类型
Plasma, Serum
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原理
- anti-EA (D)EBV IgG assay is a solid-phase immunoanalytical test.
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品牌
- ELISA-VIDITEST
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Analytical Method
- Semi-Quantitative
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组件
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- ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
- 1.3 mL Negative control human serum, r.t.u. 1) NC 1 vial
- 1.3 mL Positive control human serum, r.t.u. PC 1 vial
- 1.3 mL Calibrator (human serum), r.t.u. CAL 1 vial
- 13 mL Anti-human IgG animal antibodies labelled with horseradish peroxidase (anti-IgG Px conjugate) r.t.u. CONJ 1 vial
- 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
- 60 mL Dilution buffer, r.t.u. DIL 1 vial
- 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H2O 2) TMB 1 vial
- 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
- Instruction manual
- Quality Control Certificate
- 1) r.t.u., ready to use
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试剂未包括
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- Distilled or deionised water for dilution of buffer and standard concentrates
- appropriate and calibrated equipment for pipetting
- temperature controlled incubator
- spectrophotometer or platereader with the appropriate filters
- empty microtiter plate for pre-incubation
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
- anti-EA (D)EBV IgG assay is a solid-phase immunoanalytical test. The polystyrene strips are coated with a specific recombinant antigen containing immunodominant epitopes of EA antigen. Anti-EA (D) antibodies from samples bind to the immobilized antigens. The other serum antibodies do not bind and are washed away those that formed complexes with the antigens are later on recognized by animal anti-human IgG antibodies labelled with horseradish peroxidase. The presence of the labelled antibodies is revealed by an enzymatic reaction with a chromogenic substrate. Negative samples do not react and the mild change in colour, if present, may be attributed to the reaction background.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- EBV EA(D) IgG (Anti-Epstein-Barr Virus Early Antigen (D) IgG (EBV EA(D) IgG))
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别名
- Anti-EA (D) Epstein-Barr Virus (EBV) IgG Antibody
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物质类
- Antibody
抗原 See all EBV EA(D) IgG products
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