IgM ELISA 试剂盒
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Quick Overview for IgM ELISA 试剂盒 (ABIN457299)
抗原
See all IgM ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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最低检测浓度
- 156 ng/mL
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原理
- The IgM(pig)-ELISA is designed for the quantitative determination of pig IgM in complex samples (serum or other biological samples).
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Analytical Method
- Quantitative
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产品特性
- The determination of pig IgM is carried out as direct sandwich ELISA.
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板类型
- Pre-coated
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实验流程
- An antibody specific for pig IgM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgM present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgM concentration.
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实验精密度
- Intraassay precision (CV): (n=10) at 57 ng/ml: 5.3% at 218 ng/ml: 2.7% at 580 ng/ml: 4.7%
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限制
- 仅限研究用
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储存条件
- 4 °C
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- IgM
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物质类
- Antibody
抗原 See all IgM ELISA试剂盒
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