IgG ELISA 试剂盒
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北京 101111
Quick Overview for IgG ELISA 试剂盒 (ABIN457295)
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See all IgG ELISA试剂盒适用
检测方法
实验类型
应用范围
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最低检测浓度
- 15.6 ng/mL
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原理
- The IgG(bovine)-ELISA is designed for the quantitative determination of bovine IgG in complex samples.
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Analytical Method
- Quantitative
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特异性
- IgG (mouse) <0.1%
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产品特性
- The determination of bovine IgG is carried out as direct sandwich ELISA. Main application of the assay is to monitor purification of protein preparations (e.g. monoclonal antibodies) if bovine or calf serum has been used in the production process.
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IgG ELISA Kit
适用: 兔 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Rabbit TrueBlot® Set (with IP beads)WB, IP 适用: 兔
Mouse TrueBlot® Set (with IP beads)WB, IP 适用: 小鼠
IgG ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA Kit适用: Pig Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA Kit适用: 小鼠 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA Kit适用: 人 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA Kit适用: 小鸡 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA Kit适用: Cow Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
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板类型
- Pre-coated
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实验流程
- An antibody specific for bovine IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgG concentration.
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实验流程
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Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm -
实验精密度
- Intraassay precision (CV): (n=12) at 35 ng/ml: 2.9% at 112 ng/ml: 5.4%
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限制
- 仅限研究用
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储存条件
- 4 °C
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- IgG
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物质类
- Antibody
抗原 See all IgG ELISA试剂盒
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