Prolactin ELISA 试剂盒
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Quick Overview for Prolactin ELISA 试剂盒 (ABIN456250)
抗原
See all Prolactin (PRL) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.156 ng/mL
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原理
- This immunoassay kit allows for the in vitro quantitative determination of mouse prolactin, PRL concentrations in serum, plasma and other biological fluids.
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Analytical Method
- Quantitative
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特异性
- This assay recognizes recombinant and natural mouse prolactin.
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交叉反应 （详细）
- No significant cross-reactivity or interference was observed.
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灵敏度
- The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
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产品特性
- Mus musculus,Mouse,Prolactin,PRL,Prl
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Prolactin (PRL) ELISA Kit
PRL 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Prolactin (PRL) ELISA KitPRL 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Prolactin (PRL) ELISA KitPRL 适用: Cow Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Prolactin (PRL) ELISA KitPRL 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Prolactin (PRL) ELISA KitPRL 适用: Cow Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Plasma, Serum
Prolactin (PRL) ELISA KitPRL 适用: 小鼠 AA 32-228 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (citrate), Plasma (heparin), Serum
Prolactin (PRL) ELISA KitHigh Sensitivity PRL 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Prolactin (PRL) ELISA KitPRL 适用: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Prolactin (PRL) ELISA KitPRL 适用: 兔 Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Plasma, Serum
Prolactin (PRL) ELISA KitPRL 适用: 绵羊 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to prolactin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for prolactin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain prolactin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of prolactin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- Prolactin (PRL)
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别名
- Prl
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背景
- Prolactin (PRL) is a peptide hormone primarily associated with lactation. In breastfeeding, the infant suckling the teat stimulates the production of prolactin, which fills the breast with milk (lactogenesis) in preparation for the next feed. Oxytocin, a similar hormone, is also released, which triggers milk let-down. Prolactin (PRL) is a polypeptide hormone secreted by anterior pituitary of both male and female. Release of prolactin is controlled by a complex neuroendocrine reflex initiated by a tactile stimulus and regulated by hypothalamic releasing and inhibition factors.
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途径
- JAK/STAT Signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Protein targeting to Nucleus
抗原 See all Prolactin (PRL) ELISA试剂盒
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