Osteopontin ELISA 试剂盒
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Quick Overview for Osteopontin ELISA 试剂盒 (ABIN456061)
抗原
See all Osteopontin (SPP1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.312 ng/mL
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原理
- This immunoassay kit allows for the specific measurement of Mouse Osteopontin (OPN) concentrations in cell culture supernates, serum and plasma.
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Analytical Method
- Quantitative
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特异性
- This assay recognizes recombinant and natural mouse Osteopontin.
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交叉反应 （详细）
- No significant cross-reactivity or interference was observed.
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产品特性
- Mus musculus,Mouse,Osteopontin,2AR,Bone sialoprotein 1,Calcium oxalate crystal growth inhibitor protein,Early T-lymphocyte activation 1 protein,Minopontin,Secreted phosphoprotein 1,SPP-1,Spp1,Eta-1,Op,Spp-1
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Secreted phosphoprotein 1 (SPP1) ELISA Kit
SPP1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 小鼠 Colorimetric Sandwich ELISA 39 pg/mL - 2500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 犬 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 人 AA 17-300 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Serum, Urine
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 小鼠 AA 17-294 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Secreted phosphoprotein 1 (SPP1) ELISA KitHigh Sensitivity SPP1 适用: 犬 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 猴 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 兔 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: Pig Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Osteopontin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Osteopontin present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Osteopontin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Osteopontin bound in the initial step. The color development is stopped and the intensity of the color is measured.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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: "Proliferation and osteogenesis of immortalized bone marrow-derived mesenchymal stem cells in porous polylactic glycolic acid scaffolds under perfusion culture." in: Journal of biomedical materials research. Part A, Vol. 92, Issue 3, pp. 817-29, (2010) (PubMed).
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- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
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别名
- Spp1
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背景
- Osteopontin (OPN), also known as early T lymphocyte activation 1 (Eta-1), is a secreted multifunctional glyco-phosphoprotein with roles in bone metabolism, immune regulation, tissue remodeling , cell survival, and tumor progression . Gene structure and chromosomal location identify OPN as a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family that also includes bone sialoprotein (BSP), dentin matrix protein 1 (DMP1), dentin sialophosphoprotein(DSPP), enamelin (ENAM), and matrix extracellular phosphoglycoprotein (MEPE). Murine OPN issynthesized as a 294 amino acid (aa) precursor protein with a predicted 16 aa signal peptide and a highly unusual mature protein sequence, containing 68 acidic aa and 23 potential Ser/Thr phosphorylation sites. Although the predicted molecular weight of OPN is 31 kDa,phosphorylation and N- and O-glycosylation may allow it to appear as large as 75 kDa. Variability in post-translational modifications can influence the activity of OPN. Osteopontin, as indicated by its name meaning “bone bridging”, is highly expressed in mineralized tissues. It is also expressed in other tissues including cartilage, kidney, vascular tissues, activated macrophages, lymphocytes, and epithelia. A portion of cell expressed OPN is also retained intracellularly. In vitro, OPN stimulates the adhesion of osteoclasts to bone, and bone resorption is blocked by inhibition of this interaction. Knockout mice have outwardly normal bone development, but exhibit deficient postnatal bone resorption in several contexts, supporting a role for OPN in osteoclast function. In kidney epithelia, OPN is upregulated by high concentrations of oxalate and inhibits calcium oxalate crystal nucleation and growth. In endothelial and smooth muscle cells, OPN is upregulated by high phosphate concentration and during atherosclerosis, binding of OPN to hydroxyapatite inhibits calcification of blood vessels and heart valves. OPN expression by macrophages and T cells is upregulated by inflammatory mediators including LPS,NO, IL-1β, and TNF-α. OPN regulates macrophage differentiation and recruitment. It also functions as a chemotactic factor and co-stimulator of T cells and may act as a Th1 cytokine, stimulating IL-12 production. OPN production by macrophages is upregulated at sites of tissue remodeling including the placenta, endometrium and myocardium post-infarction. OPN is expressed by many tumor types and plays a role in tumor progression and metastasis. OPN may also be upregulated during autoimmune processes such as rheumatoid arthritis or in 2 granulomatous lesions in response to infections such as tuberculosis.
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途径
- Regulation of Cell Size
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