Osteocalcin ELISA 试剂盒
Quick Overview for Osteocalcin ELISA 试剂盒 (ABIN456053)
抗原
See all Osteocalcin (BGLAP) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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原理
- This immunoassay kit allows for the in vitro quantitative determination of mouse Osteocalcin/Bone gla protein, OT/BG concentrations in serum, plasma and other biological fluids.
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Analytical Method
- Quantitative
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特异性
- This assay recognizes recombinant and natural mouse OT.
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交叉反应 （详细）
- No significant cross-reactivity or interference was observed.
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灵敏度
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< 3.9 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero. -
产品特性
- Mus musculus,Mouse,Osteocalcin,Bone Gla protein,BGP,Gamma-carboxyglutamic acid-containing protein,Bglap
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Osteocalcin (BGLAP) ELISA Kit
BGLAP 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 小鸡 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Plasma, Serum
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 兔 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: Pig Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 大鼠 Colorimetric Sandwich ELISA 0.312 pg/mL - 20 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 猴 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Osteocalcin (BGLAP) ELISA KitHigh Sensitivity BGLAP 适用: 人 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to OT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for OT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain OT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 2 nm. The concentration of OT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- Osteocalcin (BGLAP)
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别名
- Bglap
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背景
- Osteocalcin (OT) is the most abundant noncollagenous protein in mature bone where it constitutes 1% to 2% of the total protein. Synthesized by osteoblasts, it is incorporated into the bone matrix. Osteocalcin is a 49-residue protein with three gamma-carboxyglutamic acid residues, at positions 17, 21 and 24. These three residues confer on it a very strong ability to bind to hydroxyapatite. Osteocalcin is a noncollagenous protein found in bone and dentin. It is secreted by osteoblasts and thought to play a role in mineralization and calcium ion homeostasis. It has been stipulated that osteocalcin may also function as a negative regulator of bone formation, although its exact role is unknown. It was reported that osteocalcin acts as a hormone in the body, causing beta cells in the pancreas to release more insulin, and at the same time directing fat cells to release the hormone adiponectin, which increases sensitivity to insulin. As osteocalcin is manufactured by osteoblasts, it is often used as a biochemical marker, or biomarker, for the bone formation process. It has been routinely observed that higher serum-osteocalcin levels are relatively well correlated with increases in bone mineral density (BMD) during treatment with anabolic bone formation drugs for osteoporosis, such as Forteo. In many studies, Osteocalcin is used as a preliminary biomarker on the effectiveness of a given drug on bone formation.
抗原 See all Osteocalcin (BGLAP) ELISA试剂盒
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