AGLU ELISA 试剂盒
This Colorimetric ELISA kit is designed for the quantitative measurement of 人 AGLU.
产品编号 ABIN455810
发货至:
中国
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Our Local Distributor
中国
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北京 101111
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Quick Overview for AGLU ELISA 试剂盒 (ABIN455810)
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AGLU
(alpha-Glucosidase (AGLU))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
样品类型
Tissue Homogenate
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原理
- This immunoassay kit allows for the in vitro quantitative determination of human MAL concentrations in tissue homogenates and other biological fluids.
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Analytical Method
- Quantitative
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特异性
- This assay recognizes recombinant and natural human MAL.
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交叉反应 （详细）
- No significant cross-reactivity or interference was observed.
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灵敏度
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< 0.78 U/L
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero. -
产品特性
- Homo sapiens,Human,Maltase-glucoamylase, intestinal,MGAM,MGA,MGAML
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alpha-Glucosidase (AGLU) ELISA Kit
AGLU 适用: 人 Colorimetric Sandwich ELISA 3.12-200 μU/mL Plasma, Serum, Tissue Homogenate, Urine
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MAL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MAL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MAL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- AGLU (alpha-Glucosidase (AGLU))
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别名
- MGAM
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背景
- Maltase (acid maltase, alpha-1,4-glucosidase) is an enzyme produced by the cells lining the small intestine that breaks down the disaccharide maltose. It comes under the enzyme category carbohydrase (which is a subcategory of hydrolase). Maltase is secreted by the surface cells of the villi, which are thin projections on the mucosa. These are found throughout the small intestine, but differ in shape in the duodenum and ileum sections. It must also be located in other places, like the heart, if a deficiency of it causes Pompe's Disease (which results in cardiomegaly).
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途径
- Cellular Glucan Metabolic Process
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