LIF ELISA 试剂盒
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Quick Overview for LIF ELISA 试剂盒 (ABIN455685)
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See all LIF ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- This immunoassay kit allows for the specific measurement of human LIF concentrations in cell culture supernates, serum, and plasma.
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Analytical Method
- Quantitative
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特异性
- This assay recognizes recombinant and natural human LIF.
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交叉反应 （详细）
- No significant cross-reactivity or interference was observed.
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产品特性
- Homo sapiens,Human,Leukemia inhibitory factor,LIF,Differentiation-stimulating factor,D factor,Melanoma-derived LPL inhibitor,MLPLI,LIF,HILDA
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Leukemia Inhibitory Factor (LIF) ELISA Kit
LIF 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor (LIF) ELISA KitLIF 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor (LIF) ELISA KitLIF 适用: 大鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Bronchoalveolar Lavage, Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor (LIF) ELISA KitLIF 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor (LIF) ELISA KitLIF 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor (LIF) ELISA KitLIF 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor (LIF) ELISA KitLIF 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor (LIF) ELISA KitLIF 适用: 小鸡 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor (LIF) ELISA KitLIF 适用: Cow Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor (LIF) ELISA KitLIF 适用: Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for LIF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LIF present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for LIF is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LIF bound in the initial step. The color development is stopped and the intensity of the color is measured.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- LIF (Leukemia Inhibitory Factor (LIF))
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别名
- LIF
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背景
- Leukemia Inhibitory Factor (or LIF) is a variably glycosylated, 38 - 67 kDa polypeptide originally identified as a proliferation inhibitor and differentiation inducer of the mouse M1 myeloid leukemia cell line. The mature LIF molecule measures 180 amino acid (aa) residues in length, with multiple potential N-linked and O-linked glycosylation sites plus six conserved cysteines that form three intramolecular disulfide bridges. Mature mouse LIF is 78% identical to human LIF at the aa sequence level. Although such homology might suggest a high conservation of LIF biology, notable differences exist between the reported forms of mouse and human LIF proteins and their receptors. For example, alternative splice events are known to occur in mice, but not humans, creating two isoforms of secreted LIF. Also, three isoforms of the LIF receptor a-chain, two soluble forms and one transmembrane form, have been reported in mice, but not in humans. Based on its helical structure, LIF is considered to be a member of the Interleukin-6 family of cytokines. Cells known to express LIF include activated T-cells, monocytes, and astrocytes, osteoblasts , keratinocytes, regenerating skeletal muscle, mast cells , and fibroblasts.
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途径
- JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
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