Interleukin 17a ELISA 试剂盒
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Quick Overview for Interleukin 17a ELISA 试剂盒 (ABIN455540)
抗原
See all Interleukin 17a (IL17A) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- This immunoassay kit allows for the specific measurement of mouse IL-17 concentrations in cell culture supernates, serum, and plasma.
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Analytical Method
- Quantitative
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特异性
- This assay recognizes recombinant and natural mouse IL-17.
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交叉反应 （详细）
- No significant cross-reactivity or interference was observed.
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产品特性
- Mus musculus,Mouse,Interleukin-17A,IL-17,IL-17A,Cytotoxic T-lymphocyte-associated antigen 8,CTLA-8,Il17a,Ctla8,Il17
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Interleukin 17A (IL17A) ELISA Kit
IL17A 适用: 人 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Serum, Tissue Homogenate, Urine
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 大鼠 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 小鼠 AA 22-158 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 人 AA 20-155 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 小鼠 Colorimetric Sandwich ELISA 6-600 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 大鼠 Colorimetric Sandwich ELISA 3.13-200 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 小鼠 Colorimetric Sandwich ELISA 15.63-1000 pg/mL Cell Culture Supernatant, Plasma, Serum
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-17 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for IL-17 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-17 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
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别名
- Il17a
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背景
- Interleukin 17 (IL-17, also known as IL-17A) is a 30 - 35 kDa variably glycosylated homodimeric protein that belongs to a unique family of cysteine-knot related proteins. Its sequence was originally isolated from an activated rodent hybridoma and termed CTLA-8 . It is synthesized as a 155 amino acid (aa) precursor that contains a 23 aa signal sequence and a 15 kDa, 132 aa mature segment. Although there are two intrachain disulfide bonds that create a ring reminiscent of those found in cysteine-knot proteins, the actual closed knot structure does not appear to form. IL-17 has one potential N-linked glycosylation site. Mature rat IL-17 is 61% and 60% aa identical to mouse and rat IL-17, respectively. It also shows limited aa sequence identity to other rat IL-17 family members. In particular, it shows 34%, 38%, 34% and 26%aa sequence identity to IL-17B, C, D, and E, respectively, and 49% aa sequence identity to IL-17F with which it is most homologous. While rodent and rat mature sequences show modest aa sequence identity, rat IL-17 is active on both mouse and rat cells. The cells principally known to produce IL-17 are the memory CD4+ T cells. In addition, CD8+ T cells as well as TCR+ CD4-CD8- T cells, neutrophils (PMNs) and eosinophils have also been reported to express mRNA transcripts for IL-17.
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