Haptoglobin ELISA 试剂盒
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Quick Overview for Haptoglobin ELISA 试剂盒 (ABIN455286)
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See all Haptoglobin (HP) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.78 μg/mL
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原理
- This immunoassay kit allows for the in vitro quantitative determination of bovine Hpt concentrations in serum, plasma and other biological fluids.
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Analytical Method
- Quantitative
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特异性
- This assay recognizes recombinant and natural bovine Hpt.
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交叉反应 （详细）
- No significant cross-reactivity or interference was observed.
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灵敏度
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< 0.195 μg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero. -
产品特性
- Bos taurus,Bovine,Haptoglobin,HP,Zonulin
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Haptoglobin (HP) ELISA Kit
HP 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 ng/mL - 4000 ng/mL Plasma, Serum
Haptoglobin (HP) ELISA KitHP 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 ng/mL - 2000 ng/mL Plasma, Serum
Haptoglobin (HP) ELISA KitHP 适用: 人 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Haptoglobin (HP) ELISA KitHP 适用: Cow Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Plasma, Serum
Haptoglobin (HP) ELISA KitHP 适用: 兔 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Haptoglobin (HP) ELISA KitHigh Sensitivity HP 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Haptoglobin (HP) ELISA KitHP 适用: Cow Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Haptoglobin (HP) ELISA KitHP 适用: Pig Colorimetric Sandwich ELISA 31.2 ng/mL - 2000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Haptoglobin (HP) ELISA KitHP 适用: 犬 Colorimetric Sandwich ELISA 62.5 ng/mL - 4000 ng/mL Plasma, Serum
Haptoglobin (HP) ELISA KitHP 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 ng/mL - 1000 ng/mL Plasma, Serum, Tissue Homogenate
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hpt. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Hpt. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Hpt, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Hpt in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- Haptoglobin (HP)
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别名
- HP
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背景
- Haptoglobin (Hpt) is a plasma protein with hemoglobin-binding capacity, and a plasma glycoproteins that form a stable complex with hemoglobin to aid the recycling of heme iron. It is a well-known marker of hemolysis. High haptoglobin level in plasma was associated with an increased cardiovascular risk in obese men, inflammation, atherosclerosis, and systemic sclerosis.
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途径
- Transition Metal Ion Homeostasis
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