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Haptoglobin ELISA 试剂盒

HP 适用: Cow Colorimetric Sandwich ELISA 0.78-50 μg/mL Plasma, Serum
产品编号 ABIN455286
发货至: 中国
  • 抗原 See all Haptoglobin (HP) ELISA试剂盒
    Haptoglobin (HP)
    适用
    • 7
    • 7
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    Cow
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.78-50 μg/mL
    最低检测浓度
    0.78 μg/mL
    应用范围
    ELISA
    原理
    This immunoassay kit allows for the in vitro quantitative determination of bovine Hpt concentrations in serum, plasma and other biological fluids.
    样品类型
    Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    This assay recognizes recombinant and natural bovine Hpt.
    交叉反应 (详细)
    No significant cross-reactivity or interference was observed.
    灵敏度
    < 0.195 μg/mL
    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
    产品特性
    Bos taurus,Bovine,Haptoglobin,HP,Zonulin
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hpt. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Hpt. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Hpt, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Hpt in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    限制
    仅限研究用
  • 储存条件
    4 °C/-20 °C
    储存方法
    The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
  • 抗原 See all Haptoglobin (HP) ELISA试剂盒
    Haptoglobin (HP)
    别名
    HP (HP 产品)
    别名
    HP ELISA Kit, wu:fb64e01 ELISA Kit, BP ELISA Kit, HP2ALPHA2 ELISA Kit, HPA1S ELISA Kit, HP-1 ELISA Kit, HPR ELISA Kit, Zonulin ELISA Kit, haptoglobin ELISA Kit, haptoglobin-like ELISA Kit, HP ELISA Kit, hp ELISA Kit, Hp ELISA Kit, LOC479668 ELISA Kit, LOC101102413 ELISA Kit
    背景
    Haptoglobin (Hpt) is a plasma protein with hemoglobin-binding capacity, and a plasma glycoproteins that form a stable complex with hemoglobin to aid the recycling of heme iron. It is a well-known marker of hemolysis. High haptoglobin level in plasma was associated with an increased cardiovascular risk in obese men, inflammation, atherosclerosis, and systemic sclerosis.
    途径
    Transition Metal Ion Homeostasis
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