GFAP ELISA 试剂盒
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- 抗原 See all GFAP ELISA试剂盒
- GFAP (Glial Fibrillary Acidic Protein (GFAP))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- This immunoassay kit allows for the in vitro quantitative determination of Mouse Glial Fibrillary Acidic Protein(GFAP) concentrations in cell culture supernates, serum, plasma, tissue homogenates and other biological fluids.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay recognizes recombinant and natural mouse GFAP.
- 交叉反应 (详细)
- No significant cross-reactivity or interference was observed.
- 灵敏度
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< 15.5 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero. - 产品特性
- Mus musculus,Mouse,Glial fibrillary acidic protein,GFAP,Gfap
- Featured
- Discover our best selling GFAP ELISA Kit
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- Discover our top product GFAP ELISA Kit
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to GFAP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GFAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GFAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GFAP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C
- 储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- 抗原 See all GFAP ELISA试剂盒
- GFAP (Glial Fibrillary Acidic Protein (GFAP))
- 别名
- Gfap (GFAP 产品)
- 别名
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