FSHB ELISA 试剂盒
The 人 FSHB ELISA Kit (ABIN455139) is a Colorimetric ELISA Kit designed to quantify 人 FSHB. This ELISA Kit has been cited in 1 publication.
(1 reference)
产品编号 ABIN455139
发货至:
中国
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Quick Overview for FSHB ELISA 试剂盒 (ABIN455139)
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FSHB
(Follicle Stimulating Hormone, beta Polypeptide (FSHB))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
1.56-100 MioU/mL
应用范围
ELISA
样品类型
Serum, Plasma
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最低检测浓度
- 1.56 MioU/mL
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原理
- This immunoassay kit allows for the specific measurement of Human Follicle-Stimulating Hormone (FSH) concentrations in serum and plasma.
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Analytical Method
- Quantitative
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特异性
- This assay recognizes recombinant and natural Human FSH.
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交叉反应 （详细）
- No significant cross-reactivity or interference was observed.
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灵敏度
- The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
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产品特性
- Homo sapiens,Human,Follitropin subunit beta,Follicle-stimulating hormone beta subunit,FSH-B,FSH-beta,Follitropin beta chain,FSHB
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Follicle Stimulating Hormone, beta Polypeptide (FSHB) ELISA Kit
FSHB 适用: 小鼠 Colorimetric Sandwich ELISA 0.94 ng/mL - 60 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Follicle Stimulating Hormone, beta Polypeptide (FSHB) ELISA KitFSHB 适用: 大鼠 Colorimetric Sandwich ELISA 0.156-10 MioU/mL Plasma, Serum
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for FSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FSH present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for FSH is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color 2 develops in proportion to the amount of FSH bound in the initial step. The color development is stopped and the intensity of the color is measured.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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: "Effects of quinestrol on reproductive hormone expression, secretion, and receptor levels in female Mongolian gerbils (Meriones unguiculatus)." in: Theriogenology, Vol. 77, Issue 6, pp. 1223-31, (2012) (PubMed).
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- FSHB (Follicle Stimulating Hormone, beta Polypeptide (FSHB))
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别名
- FSHB
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背景
- Follicle stimulating hormone (FSH) is a hormone synthesised and secreted by gonadotropes in the anterior pituitary gland. In the ovary FSH stimulates the growth of immature Graafian follicles to maturation. FSH is a glycoprotein secreted by the basophil cells of the anterior pituitary. Gonadotropin-releasing hormone (GnRH), produced in the hypothalamus, controls the release of FSH from the anterior pituitary. Like other glycoproteins, such as LH, TSH, and HCG, FSH consists of subunits designated as alpha and beta. Hormones of this type have alpha subunits that are very similar in structure, therefore the biological and immunological properties of each hormone are dependent on the unique beta subunit. In the female, FSH stimulates the growth and maturation of ovarian follicles by acting directly on the receptors located on the granulosa cells, follicular steroidogenesis is promoted and LH production is stimulated. The LH produced then binds to the theca cells and stimulates steroidogenesis. Increased intraovarian estradiol production occurs as follicular maturation advances, thereupon stimulating increased FSH receptor activity and FSH follicular binding. FSH, LH, and estradiol are therefore intimately related in supporting ovarian recruitment and maturation in women.FSH levels are elevated after menopause, castration, and in premature ovarian failure. The levels of FSH may be normalized through the administration of estrogens, which demonstrate a negative feedback mechanism. Abnormal relationships between FSH and LH, and between FSH and estrogen have been linked to anorexia nervosa and polycystic ovarian disease. Although there are significant exceptions, ovarian failure is indicated when random FSH concentrations exceed 40 mIU/mL. The growth of the seminiferous tubules and maintenance of spermatogenesis in men are regulated by FSH. However, androgens, unlike estrogens, do not lower FSH levels, therefore demonstrating a feedback relationship only with serum LH.
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途径
- Peptide Hormone Metabolism, Hormone Activity, C21-Steroid Hormone Metabolic Process
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