LDHD ELISA 试剂盒
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Quick Overview for LDHD ELISA 试剂盒 (ABIN454979)
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See all LDHD ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- This immunoassay kit allows for the in vitro quantitative determination of Mouse D-LDH, concentrations in serum, tissue homogenates and other biological fluids.
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Analytical Method
- Quantitative
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特异性
- This assay recognizes recombinant and natural Mouse D-LDH.
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交叉反应 （详细）
- No significant cross-reactivity or interference was observed.
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灵敏度
- The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
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产品特性
- Mus musculus,Mouse,Probable D-lactate dehydrogenase, mitochondrial,DLD,Lactate dehydrogenase D,Ldhd,1.1.2.4
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Lactate Dehydrogenase D (LDHD) ELISA Kit
LDHD 适用: 小鼠 Colorimetric Sandwich ELISA 4.69 mU/mL - 300 mU/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Lactate Dehydrogenase D (LDHD) ELISA KitLDHD 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Lactate Dehydrogenase D (LDHD) ELISA KitLDHD 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to D-LDH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for D-LDH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain D-LDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of D-LDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- LDHD (Lactate Dehydrogenase D (LDHD))
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别名
- Ldhd
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途径
- Ribonucleoside Biosynthetic Process
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