HMGB4 ELISA 试剂盒
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Quick Overview for HMGB4 ELISA 试剂盒 (ABIN454716)
抗原
See all HMGB4 products适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.156 ng/mL
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原理
- This immunoassay kit allows for the in vitro quantitative determination of human chondroitin sulfatase,CS concentrations in cell culture supernates, serum, plasma and other biological fluids.
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Analytical Method
- Quantitative
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特异性
- This assay recognizes recombinant and natural human CS.
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交叉反应 （详细）
- No significant cross-reactivity or interference was observed.
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灵敏度
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< 0.312 U/L
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero. -
产品特性
- Homo sapiens,Human,High mobility group protein B4,HMGB4
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High Mobility Group Box 4 (HMGB4) ELISA Kit
HMGB4 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
High Mobility Group Box 4 (HMGB4) ELISA KitHMGB4 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to CS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- HMGB4 (High Mobility Group Box 4 (HMGB4))
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别名
- HMGB4
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背景
- In enzymology, a chondroitin sulfatase is an enzyme that catalyzes the chemical reaction of cleaving off the 6-sulfate groups of the N-acetyl-D-galactosamine 6-sulfate units of the macromolecule chondroitin sulfate and, similarly, of the D-galactose 6-sulfate units of the macromolecule keratan sulfate.This enzyme belongs to the family of hydrolases, specifically those acting on sulfuric ester bonds.
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