Cholecystokinin ELISA 试剂盒
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- 抗原 See all Cholecystokinin (CCK) ELISA试剂盒
- Cholecystokinin (CCK)
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- This immunoassay kit allows for the in vitro quantitative determination of Mouse Cholecystokinin,CCK, concentrations in serum, tissue homogenates and other biological fluids.
- 样品类型
- Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay recognizes recombinant and natural mouse CCK.
- 交叉反应 (详细)
- No significant cross-reactivity or interference was observed.
- 灵敏度
- The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
- 产品特性
- Mus musculus,Mouse,Cholecystokinin,CCK,Cck
- Featured
- Discover our best selling CCK ELISA Kit
- Top Product
- Discover our top product CCK ELISA Kit
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to CCK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CCK and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CCK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CCK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C
- 储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
-
- 抗原 See all Cholecystokinin (CCK) ELISA试剂盒
- Cholecystokinin (CCK)
- 别名
- Cck (CCK 产品)
- 别名
- CCK ELISA Kit, CCK-CH ELISA Kit, cck-a ELISA Kit, cck-b ELISA Kit, cholecystokinin ELISA Kit, cholecystokinin L homeolog ELISA Kit, CCK ELISA Kit, Cck ELISA Kit, cck ELISA Kit, cck.L ELISA Kit
- 途径
- TCR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades, Feeding Behaviour
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