Aquaporin 1 ELISA 试剂盒
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Quick Overview for Aquaporin 1 ELISA 试剂盒 (ABIN454453)
抗原
See all Aquaporin 1 (AQP1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.156 ng/mL
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原理
- This immunoassay kit allows for the specific measurement of human AQP1 concentrations in cell culture supernates, tissue homogenates serum, and plasma.
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Analytical Method
- Quantitative
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特异性
- This assay recognizes recombinant and natural human AQP1.
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交叉反应 （详细）
- No significant cross-reactivity or interference was observed.
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灵敏度
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< 0.04 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero. -
产品特性
- Homo sapiens,Human,Aquaporin-1,AQP-1,Aquaporin-CHIP,Urine water channel,Water channel protein for red blood cells and kidney proximal tubule,AQP1,CHIP28
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Aquaporin 1 (Colton Blood Group) (AQP1) ELISA Kit
AQP1 适用: 人 Colorimetric Sandwich ELISA 0.25 ng/mL - 16 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Aquaporin 1 (Colton Blood Group) (AQP1) ELISA KitAQP1 适用: 大鼠 Colorimetric Sandwich ELISA 0.25 ng/mL - 16 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Aquaporin 1 (Colton Blood Group) (AQP1) ELISA KitAQP1 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Aquaporin 1 (Colton Blood Group) (AQP1) ELISA KitAQP1 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Aquaporin 1 (Colton Blood Group) (AQP1) ELISA KitAQP1 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Aquaporin 1 (Colton Blood Group) (AQP1) ELISA KitAQP1 适用: 人 Colorimetric Sandwich ELISA 0.25 ng/mL - 16 ng/mL Plasma, Serum, Tissue Homogenate
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for AQP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AQP1 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for AQP1 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AQP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))
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别名
- AQP1
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背景
- AQP1 is a polypeptide hormone produced in pancreatic beta-cells that belongs to the family of calcitonin gene-related peptides. There is a 20% sequence homology between AQP1 and calcitonin and 44% homology with calcitonin gene-related peptide. AQP1 and its fragments stimulate the proliferation of osteoblasts, inhibit bone resorption, and increase bone density and the amount of bone mass.
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途径
- Hormone Transport
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