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- 抗原 See all IgM ELISA试剂盒
- IgM
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适用
- 小鸡
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 最低检测浓度
- 98 ng/mL
- 应用范围
- ELISA
- 原理
- The IgM(chicken)-ELISA is designed for the quantitative determination of chicken IgM in complex samples (serum or other biological samples).
- 样品类型
- Serum
- Analytical Method
- Quantitative
- 产品特性
- The determination of chicken IgM is carried out as direct sandwich ELISA.
- Featured
- Discover our best selling IgM ELISA Kit
- Top Product
- Discover our top product IgM ELISA Kit
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IgM ELISA Kit
适用: 小鸡 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
IgM ELISA Kit适用: 人 Colorimetric Sandwich ELISA 31.2 ng/mL - 2000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgM ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgM ELISA Kit适用: Grouper (Epinephelinae) Colorimetric Competition ELISA 1.25-50 μg/mL Plasma, Serum, Tissue Homogenate
IgM ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 25 ng/mL - 800 ng/mL Plasma, Serum, Urine
IgM ELISA Kit适用: 大鼠 Colorimetric Sandwich ELISA 31.25 ng/mL - 1000 ng/mL Plasma, Serum, Urine
IgM ELISA Kit适用: 人 Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Plasma, Serum, Urine
IgM ELISA Kit适用: 犬 Colorimetric Sandwich ELISA 25 ng/mL - 800 ng/mL Plasma, Serum, Urine
IgM ELISA Kit适用: Cow Colorimetric Competition ELISA 0.39 μg/mL - 25 μg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
IgM ELISA Kit适用: 大鼠 Colorimetric Competition ELISA 246.9 ng/mL - 20000 ng/mL Plasma, Serum
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- 板类型
- Pre-coated
- 实验流程
- An antibody specific for chicken IgM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgM present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgM concentration.
- 实验流程
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Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm - 实验精密度
- Intraassay precision (CV):(n=10)at 7.4 ng/ml: 3.1%at 43.8 ng/ml: 6.1%at 147.3 ng/ml: 11.3%
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原 See all IgM ELISA试剂盒
- IgM
- Abstract
- IgM 产品
- 别名
- IgM constant region ELISA Kit, IGM ELISA Kit
- 物质类
- Antibody
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