IgG ELISA 试剂盒
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Quick Overview for IgG ELISA 试剂盒 (ABIN454029)
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See all IgG ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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最低检测浓度
- 156 ng/mL
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原理
- The IgG(rat)-ELISA is designed for the quantitative determination of rat IgG in complex samples (serum or other biological samples).
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Analytical Method
- Quantitative
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产品特性
- The determination of rat IgG is carried out as direct sandwich ELISA.
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IgG ELISA Kit
适用: 兔 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Rabbit TrueBlot® Set (with IP beads)WB, IP 适用: 兔
Mouse TrueBlot® Set (with IP beads)WB, IP 适用: 小鼠
IgG ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA Kit适用: Pig Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA Kit适用: 小鼠 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA Kit适用: 人 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA Kit适用: 小鸡 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA Kit适用: Cow Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
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板类型
- Pre-coated
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实验流程
- An antibody specific for rat IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgG concentration.
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实验流程
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Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm -
实验精密度
- Intraassay precision (CV): (n=10) at 73 ng/ml: 5.8% at 274 ng/ml: 8.9%
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限制
- 仅限研究用
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储存条件
- 4 °C
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- IgG
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物质类
- Antibody
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