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- 抗原 See all IgG ELISA试剂盒
- IgG
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适用
- 兔
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 最低检测浓度
- 156 ng/mL
- 应用范围
- ELISA
- 原理
- The IgG(rabbit)-ELISA is designed for the quantitative determination of rabbit IgG in complex samples (serum or other biological samples).
- 样品类型
- Serum
- Analytical Method
- Quantitative
- 产品特性
- The determination of rabbit IgG is carried out as direct sandwich ELISA.
- Featured
- Discover our best selling IgG ELISA Kit
- Top Product
- Discover our top product IgG ELISA Kit
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IgG ELISA Kit
适用: 兔 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA Kit适用: 小鼠 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA Kit适用: 人 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA Kit适用: 小鸡 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA Kit适用: 大鼠 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA Kit适用: Pig Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA Kit适用: Cow Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
Rabbit TrueBlot® Set (with IP beads)WB, IP 适用: 兔
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- 板类型
- Pre-coated
- 实验流程
- An antibody specific for rabbit IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgG concentration.
- 实验流程
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Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm - 实验精密度
- Intraassay precision (CV):(n=10)at 4.1 ng/ml: 2.9%at 17.3 ng/ml: 2.5%at 34.7 ng/ml: 2.6%
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原 See all IgG ELISA试剂盒
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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