IgY ELISA 试剂盒
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Quick Overview for IgY ELISA 试剂盒 (ABIN454026)
抗原
See all IgY products适用
检测方法
实验类型
应用范围
样品类型
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最低检测浓度
- 80 ng/mL
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原理
- The IgG(chicken)-ELISA is designed for the quantitative determination of chicken IgG in complex samples (serum or other biological samples).
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Analytical Method
- Quantitative
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产品特性
- The determination of chicken IgG is carried out as direct sandwich ELISA.
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IgY ELISA Kit
适用: 小鸡 Colorimetric Sandwich ELISA 0.078 ng/mL - 5 ng/mL Egg yolk, Plasma, Serum
IgY ELISA Kit适用: 小鸡 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
IgY ELISA Kit适用: 小鸡 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Urine
IgY ELISA Kit适用: 小鸡 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- An antibody specific for chicken IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgG concentration.
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实验流程
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Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm -
实验精密度
- Intraassay precision (CV):(n=10)at 50.8 ng/ml: 3.0%at 122.6 ng/ml: 6.6%
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限制
- 仅限研究用
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储存条件
- 4 °C
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- IgY
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别名
- IgG/IgY
抗原 See all IgY products
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