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- 抗原 See all IgY products
- IgY
- 适用
- 小鸡
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 最低检测浓度
- 80 ng/mL
- 应用范围
- ELISA
- 原理
- The IgG(chicken)-ELISA is designed for the quantitative determination of chicken IgG in complex samples (serum or other biological samples).
- 样品类型
- Serum
- Analytical Method
- Quantitative
- 产品特性
- The determination of chicken IgG is carried out as direct sandwich ELISA.
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IgY ELISA Kit
适用: 小鸡 Colorimetric Sandwich ELISA 0.078 ng/mL - 5 ng/mL Egg yolk, Plasma, Serum
IgY ELISA Kit适用: 小鸡 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Urine
IgY ELISA Kit适用: 小鸡 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
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- 板类型
- Pre-coated
- 实验流程
- An antibody specific for chicken IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgG concentration.
- 实验流程
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Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm - 实验精密度
- Intraassay precision (CV):(n=10)at 50.8 ng/ml: 3.0%at 122.6 ng/ml: 6.6%
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原 See all IgY products
- IgY
- 别名
- IgG/IgY (IgY 产品)
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