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IgA ELISA 试剂盒

适用: 大鼠 Colorimetric Sandwich ELISA Serum
产品编号 ABIN454025
发货至: 中国
  • 抗原 See all IgA ELISA试剂盒
    IgA
    适用
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    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    最低检测浓度
    156 ng/mL
    应用范围
    ELISA
    原理
    The IgA(rat)-ELISA is designed for the quantitative determination of rat IgA in complex samples (serum or other biological samples).
    样品类型
    Serum
    Analytical Method
    Quantitative
    产品特性
    The determination of rat IgA is carried out as direct sandwich ELISA.
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  • 板类型
    Pre-coated
    实验流程
    An antibody specific for rat IgA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgA present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgA concentration.
    实验流程

    Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
    Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
    Wash Substrate incubation: 100 µL/well 10 min, room temperature
    Add stop solution: 100 µL/well
    Read at 450 nm

    实验精密度
    Intraassay precision (CV):(n=10)at 43 ng/ml: 3.8%at 99 ng/ml: 6.9%
    限制
    仅限研究用
  • 储存条件
    4 °C
  • 抗原 See all IgA ELISA试剂盒
    IgA
    Abstract
    IgA 产品
    别名
    MB-1 ELISA Kit, IG-alpha ELISA Kit, IGA ELISA Kit, IgA ELISA Kit, Igh-2 ELISA Kit, CD79a molecule ELISA Kit, immunoglobulin heavy constant alpha ELISA Kit, CD79A ELISA Kit, Igha ELISA Kit
    物质类
    Antibody
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