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Anti-Beta2 Adrenergic Receptor Antibodies (IgG) ELISA 试剂盒

This Colorimetric ELISA kit is designed for the quantitative measurement of 人 Anti-Beta2 Adrenergic Receptor Antibodies (IgG).
产品编号 ABIN454022
发货至: 中国
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Quick Overview for Anti-Beta2 Adrenergic Receptor Antibodies (IgG) ELISA 试剂盒 (ABIN454022)

抗原

Anti-Beta2 Adrenergic Receptor Antibodies (IgG)

适用

检测方法

Colorimetric

实验类型

Competition ELISA

应用范围

ELISA
  • 原理

    The anti-beta2-adrenergic-receptor-antibody-EIA is an antibody screening test. The beta2-adrenergic-receptor has been pre-coated onto a microtiter plate. During the first incubation the anti-beta2-adrenergic-receptor-antibodies of the samples are immobilised on the plate. The auto-antibodies are detected with a POD labeled anti-human IgG antibody. In the following enzymatic substrate reaction the intensity of the colour correlates with the concentration and/ or avidity of anti-beta2-adrenergic-receptor-antibodies.

    Analytical Method

    Quantitative
  • 板类型

    Pre-coated

    实验流程

    Incubation of samples/ standards/ controls: 100 µL 120 min, 4°C
    Wash Incubation of detection antibody: 100 µL 1 hr, room temperature
    Wash Substrate incubation: 100 µL/well 20 min, room temperature
    Add Stopp solution: 100 µL/well
    Read at 450nm

    实验精密度

    Intraassay-Precision:7.34% Interassay-Precision:10.82%

    限制

    仅限研究用
  • 储存条件

    4 °C
  • 抗原

    Anti-Beta2 Adrenergic Receptor Antibodies (IgG)

    物质类

    Antibody

    背景

    The beta-2 adrenergic receptor is a G protein-coupled receptor. It consists of three highly homologous subtypes, including a1A-, a1B-, and a1D-adrenergic. The anti-(2-adrenergic-receptor-antibody-EIA is designed for the determination of Antibodies (IgG) against the (2-adrenergic-receptor in serum and plasma.
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