Anti-Alpha2 Adrenergic Receptor Antibodies (IgG) ELISA 试剂盒
人 Anti-Alpha2 Adrenergic Receptor Antibodies (IgG) ELISA Kit, Colorimetric is an assay for quantification of 人 Anti-Alpha2 Adrenergic Receptor Antibodies (IgG).
产品编号 ABIN454021
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Anti-Alpha2 Adrenergic Receptor Antibodies (IgG) ELISA 试剂盒 (ABIN454021)
抗原
Anti-Alpha2 Adrenergic Receptor Antibodies (IgG)
适用
人
检测方法
Colorimetric
实验类型
Competition ELISA
应用范围
ELISA
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原理
- The anti-alpha2-adrenergic-receptor-antibody-EIA is an antibody screening test. The alpha2-adrenergic-receptor has been pre-coated onto a microtiter plate. During the first incubation the anti-alpha2-adrenergic-receptor-antibodies of the samples are immobilised on the plate. The auto-antibodies are detected with a POD labeled anti-human IgG antibody. In the following enzymatic substrate reaction the intensity of the colour correlates with the concentration and/ or avidity of anti-alpha2-adrenergic-receptor-antibodies.
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Analytical Method
- Quantitative
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板类型
- Pre-coated
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实验流程
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Incubation of samples/ standards/ controls: 100 µL 120 min, 4°C
Wash Incubation of detection antibody: 100 µL 1 hr, room temperature
Wash Substrate incubation: 100 µL/well 20 min, room temperature
Add Stopp solution: 100 µL/well
Read at 450nm -
实验精密度
- Intraassay-Precision:9,12% Interassay-Precision:13.44%
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限制
- 仅限研究用
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储存条件
- 4 °C
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- Anti-Alpha2 Adrenergic Receptor Antibodies (IgG)
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物质类
- Antibody
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背景
- The Alpha-2 adrenergic receptor is a G protein-coupled receptor. ß receptors have the subtypes ß1, ß2 and ß3. Agonist binding causes a rise in the intracellular concentration of the second messenger cAMP. The anti-alpha2-adrenergic-receptor-antibody-EIA is designed for the determination of Antibodies (IgG) against the Alpha2-adrenergic-receptor in serum and plasma.
抗原
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