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Albumin ELISA 试剂盒

ALB 适用: Cow Colorimetric Competition ELISA Urine
产品编号 ABIN454014
发货至: 中国
  • 抗原 See all Albumin (ALB) ELISA试剂盒
    Albumin (ALB)
    适用
    • 10
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    Cow
    检测方法
    Colorimetric
    实验类型
    Competition ELISA
    最低检测浓度
    50 ng/mL
    应用范围
    ELISA
    原理
    The ALBUMIN(bovine)-ELISA is designed for the quantitative determination of bovine albumin in complex samples.
    样品类型
    Urine
    Analytical Method
    Quantitative
    特异性
    Albumin (human) <0.1%Albumin (mouse) <0.1%
    产品特性
    The determination of albumin is carried out as direct competitive ELISA. Main application of the assay is to monitor purification of protein preparations (e.g. monoclonal antibodies) if bovine or calf serum albumin has been used in the production process. This ELISA furthermore allows quantification of albumin in bovine urine to estimate kidney function. When blood is filtered in glomeruli of kidneys, highly molecular substances are held back. Therefore, urine is nearly protein free. In case of kidney damages, small proteins like albumin appear in urine. Albumin therefore serves as a marker in monitoring kidney damages.
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  • 板类型
    Pre-coated
    实验流程
    Bovine albumin has been pre-coated onto a microplate. During incubation the binding of an enzyme-linked anti-albumin(bovine)-antibody to the wells is inhibited by albumin from samples/standards. After washing away any unbound antibody, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is reverse proportional to the albumin concentration.
    实验流程

    Incubation of samples/standards with antibody conjugate: 50 µL + 50 µL/well 2 hrs, room temperature.
    Wash Substrate incubation: 100 µL/well 20 min, room temperature.
    Add stop solution: 100 µL/well.
    Read at 450 nm

    实验精密度
    Intraassay precision (CV):(n=10)at 2529 ng/ml: 5.1%at 875 ng/ml: 8.8%at 39 ng/ml: 10.7% Recovery:106.6%
    限制
    仅限研究用
  • 储存条件
    4 °C
  • 抗原 See all Albumin (ALB) ELISA试剂盒
    Albumin (ALB)
    别名
    Albumin (ALB 产品)
    别名
    PRO0883 ELISA Kit, PRO0903 ELISA Kit, PRO1341 ELISA Kit, ALB ELISA Kit, CSA ELISA Kit, Alb-1 ELISA Kit, Alb1 ELISA Kit, Albza ELISA Kit, LOC100136344 ELISA Kit, alb-a ELISA Kit, alb-b ELISA Kit, albumin ELISA Kit, serum albumin 1 ELISA Kit, albumin S homeolog ELISA Kit, ALB ELISA Kit, Alb ELISA Kit, LOC100136344 ELISA Kit, alb.S ELISA Kit
    途径
    Lipid Metabolism
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