Albumin ELISA 试剂盒
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- 抗原 See all Albumin (ALB) ELISA试剂盒
- Albumin (ALB)
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适用
- Cow
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 最低检测浓度
- 50 ng/mL
- 应用范围
- ELISA
- 原理
- The ALBUMIN(bovine)-ELISA is designed for the quantitative determination of bovine albumin in complex samples.
- 样品类型
- Urine
- Analytical Method
- Quantitative
- 特异性
- Albumin (human) <0.1%Albumin (mouse) <0.1%
- 产品特性
- The determination of albumin is carried out as direct competitive ELISA. Main application of the assay is to monitor purification of protein preparations (e.g. monoclonal antibodies) if bovine or calf serum albumin has been used in the production process. This ELISA furthermore allows quantification of albumin in bovine urine to estimate kidney function. When blood is filtered in glomeruli of kidneys, highly molecular substances are held back. Therefore, urine is nearly protein free. In case of kidney damages, small proteins like albumin appear in urine. Albumin therefore serves as a marker in monitoring kidney damages.
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- 板类型
- Pre-coated
- 实验流程
- Bovine albumin has been pre-coated onto a microplate. During incubation the binding of an enzyme-linked anti-albumin(bovine)-antibody to the wells is inhibited by albumin from samples/standards. After washing away any unbound antibody, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is reverse proportional to the albumin concentration.
- 实验流程
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Incubation of samples/standards with antibody conjugate: 50 µL + 50 µL/well 2 hrs, room temperature.
Wash Substrate incubation: 100 µL/well 20 min, room temperature.
Add stop solution: 100 µL/well.
Read at 450 nm - 实验精密度
- Intraassay precision (CV):(n=10)at 2529 ng/ml: 5.1%at 875 ng/ml: 8.8%at 39 ng/ml: 10.7% Recovery:106.6%
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原 See all Albumin (ALB) ELISA试剂盒
- Albumin (ALB)
- 别名
- Albumin (ALB 产品)
- 别名
- PRO0883 ELISA Kit, PRO0903 ELISA Kit, PRO1341 ELISA Kit, ALB ELISA Kit, CSA ELISA Kit, Alb-1 ELISA Kit, Alb1 ELISA Kit, Albza ELISA Kit, LOC100136344 ELISA Kit, alb-a ELISA Kit, alb-b ELISA Kit, albumin ELISA Kit, serum albumin 1 ELISA Kit, albumin S homeolog ELISA Kit, ALB ELISA Kit, Alb ELISA Kit, LOC100136344 ELISA Kit, alb.S ELISA Kit
- 途径
- Lipid Metabolism
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