Albumin ELISA 试剂盒
Quick Overview for Albumin ELISA 试剂盒 (ABIN454014)
抗原
See all Albumin (ALB) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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最低检测浓度
- 50 ng/mL
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原理
- The ALBUMIN(bovine)-ELISA is designed for the quantitative determination of bovine albumin in complex samples.
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Analytical Method
- Quantitative
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特异性
- Albumin (human) <0.1%Albumin (mouse) <0.1%
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产品特性
- The determination of albumin is carried out as direct competitive ELISA. Main application of the assay is to monitor purification of protein preparations (e.g. monoclonal antibodies) if bovine or calf serum albumin has been used in the production process. This ELISA furthermore allows quantification of albumin in bovine urine to estimate kidney function. When blood is filtered in glomeruli of kidneys, highly molecular substances are held back. Therefore, urine is nearly protein free. In case of kidney damages, small proteins like albumin appear in urine. Albumin therefore serves as a marker in monitoring kidney damages.
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Albumin (ALB) ELISA Kit
ALB 适用: 人 Colorimetric Competition ELISA 246.9 ng/mL - 20000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Albumin (ALB) ELISA KitALB 适用: 小鼠 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 大鼠 Colorimetric Competition ELISA 123.5 ng/mL - 10000 ng/mL Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 大鼠 Colorimetric Sandwich ELISA 3.13 μg/mL - 200 μg/mL Cell Culture Supernatant, Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 大鼠 Colorimetric Sandwich ELISA 7.813 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate
Albumin (ALB) ELISA KitHigh Sensitivity ALB 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 犬 Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
Albumin (ALB) ELISA KitALB 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Urine
Albumin (ALB) ELISA KitALB 适用: 兔 Colorimetric Competition ELISA 617.3 ng/mL - 50000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Bovine albumin has been pre-coated onto a microplate. During incubation the binding of an enzyme-linked anti-albumin(bovine)-antibody to the wells is inhibited by albumin from samples/standards. After washing away any unbound antibody, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is reverse proportional to the albumin concentration.
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实验流程
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Incubation of samples/standards with antibody conjugate: 50 µL + 50 µL/well 2 hrs, room temperature.
Wash Substrate incubation: 100 µL/well 20 min, room temperature.
Add stop solution: 100 µL/well.
Read at 450 nm -
实验精密度
- Intraassay precision (CV):(n=10)at 2529 ng/ml: 5.1%at 875 ng/ml: 8.8%at 39 ng/ml: 10.7% Recovery:106.6%
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限制
- 仅限研究用
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储存条件
- 4 °C
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- Albumin (ALB)
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别名
- Albumin
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途径
- Lipid Metabolism
抗原 See all Albumin (ALB) ELISA试剂盒
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