Albumin ELISA 试剂盒
(5 references)Quick Overview for Albumin ELISA 试剂盒 (ABIN454013)
抗原
See all Albumin (ALB) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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最低检测浓度
- 0.1 µg/mL
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原理
- This ELISA allows quantification of albumin in mouse urine, so that an estimation of kidney function is possible.
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Analytical Method
- Quantitative
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产品特性
- Determination of albumin is carried out as direct competitive ELISA.
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Albumin (ALB) ELISA Kit
ALB 适用: 人 Colorimetric Competition ELISA 246.9 ng/mL - 20000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Albumin (ALB) ELISA KitALB 适用: 小鼠 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 大鼠 Colorimetric Competition ELISA 123.5 ng/mL - 10000 ng/mL Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 大鼠 Colorimetric Sandwich ELISA 3.13 μg/mL - 200 μg/mL Cell Culture Supernatant, Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 大鼠 Colorimetric Sandwich ELISA 7.813 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate
Albumin (ALB) ELISA KitHigh Sensitivity ALB 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 犬 Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
Albumin (ALB) ELISA KitALB 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Urine
Albumin (ALB) ELISA KitALB 适用: 兔 Colorimetric Competition ELISA 617.3 ng/mL - 50000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Mouse albumin has been pre-coated onto a microtiter plate. During incubation binding of an enzyme-linked anti-albumin(mouse)-antibody is inhibited by albumin from samples/ standards. After washing away any unbound antibody a substrate solution is added. Developing colour correlated with the amount of antibody conjugate. Absorption at 450 nm is reverse proportional to albumin concentration.
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实验流程
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Incubation of samples/standards with antibody conjugate: 50 µL + 50 µL/well 90 min with shaking,120 min without shaking.
Wash Substrate incubation: 100 µL/well 10 min, room temperature.
Add stop solution 100 µL/well.
Read at 450 nm -
实验精密度
- Intraassay precision (CV):(sample dilution 1:100, n=12) at 3.1 µg/ml: 14.3%at 30.4 µg/ml: 6.1% at 223 µg/ml: 5.8%
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限制
- 仅限研究用
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储存条件
- 4 °C
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: "Growth arrest specific 2-like protein 1 expression is upregulated in podocytes through advanced glycation end-products." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, (2016) (PubMed).
: "OPN deficiency results in severe glomerulosclerosis in uninephrectomized mice." in: American journal of physiology. Renal physiology, (2013) (PubMed).
: "An Ethanolic Extract of Lindera obtusiloba Stems, YJP-14, Improves Endothelial Dysfunction, Metabolic Parameters and Physical Performance in Diabetic db/db Mice." in: PLoS ONE, Vol. 8, Issue 6, pp. e65227, (2013) (PubMed).
: "Lipid phosphatase SHIP2 downregulates insulin signalling in podocytes." in: Molecular and cellular endocrinology, Vol. 328, Issue 1-2, pp. 70-9, (2010) (PubMed).
: "beta-Catenin mediates adriamycin-induced albuminuria and podocyte injury in adult mouse kidneys." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 25, Issue 8, pp. 2437-46, (2010) (PubMed).
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: "Growth arrest specific 2-like protein 1 expression is upregulated in podocytes through advanced glycation end-products." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, (2016) (PubMed).
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- Albumin (ALB)
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别名
- Albumin
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背景
- When blood is filtered in glomeruli of kidneys, highly molecular substances are held back. Therefore, urine is nearly protein free. In case of kidney damages, small proteins like albumin appear in urine. Albumin therefore serves as a marker in monitoring kidney damages. In examining nephrotoxic substances and therapeutic strategies regarding kidney damages, animal models are frequently used.
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途径
- Lipid Metabolism
抗原 See all Albumin (ALB) ELISA试剂盒
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