(6 references)-
- 抗原 See all Albumin (ALB) ELISA试剂盒
- Albumin (ALB)
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 最低检测浓度
- 50 ng/mL
- 应用范围
- ELISA
- 原理
- The Alpha2-macroglobulin (rat)-ELISA is designed for the quantitative determination of Alpha2-macroglobulin (A2M) in rat body fluids and cell culture supernatants.
- 样品类型
- Cell Culture Supernatant
- Analytical Method
- Quantitative
- 产品特性
- The determination of A2M is carried out as competitive ELISA.
- Featured
- Discover our best selling ALB ELISA Kit
- Top Product
- Discover our top product ALB ELISA Kit
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Albumin (ALB) ELISA Kit
ALB 适用: 大鼠 Colorimetric Competition ELISA 123.5 ng/mL - 10000 ng/mL Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 小鼠 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 人 Colorimetric Competition ELISA 246.9 ng/mL - 20000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Albumin (ALB) ELISA KitALB 适用: 大鼠 Colorimetric Sandwich ELISA 3.13 μg/mL - 200 μg/mL Cell Culture Supernatant, Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 大鼠 Colorimetric Sandwich ELISA 7.813 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate
Albumin (ALB) ELISA KitALB 适用: 豚鼠 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Urine
Albumin (ALB) ELISA KitHigh Sensitivity ALB 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Plasma, Serum, Urine
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- 板类型
- Pre-coated
- 实验流程
- A2M has been pre-coated onto a microplate. During incubation the binding of an anti-A2M(rat)-antibody to the wells is inhibited by A2M from samples/standards. The immobilized antibody is detected using a HRP-labelled secondary antibody. After washing away any unbound antibody, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is reverse proportional to the A2M concentration.
- 实验流程
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Incubation of samples/standards with antibody conjugate: 50 µL + 50 µL/well 60 min, room temperature with shaking
Wash Incubation of detection antibody (HRP-labeled): 100 µL/well 60 min with shaking
Wash Substrate incubation: 100 µL/well 20 min, room temperature
Add stop solution: 100 µL/well Read at 450 nm - 限制
- 仅限研究用
-
- 储存条件
- 4 °C
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: "Tubulointerstitial De Novo Expression of the α8 Integrin Chain in a Rodent Model of Renal Fibrosis - A Potential Target for Anti-Fibrotic Therapy?" in: PLoS ONE, Vol. 7, Issue 11, pp. e48362, (2012) (PubMed).
: "Angiotensin II Type 1 Receptor Antibodies and Increased Angiotensin II Sensitivity in Pregnant Rats." in: Hypertension, Vol. 58, Issue 1, pp. 77-84, (2011) (PubMed).
: "Lipid phosphatase SHIP2 downregulates insulin signalling in podocytes." in: Molecular and cellular endocrinology, Vol. 328, Issue 1-2, pp. 70-9, (2010) (PubMed).
: "Lack of {alpha}8 integrin aggravates podocyte injury in experimental diabetic nephropathy." in: American journal of physiology. Renal physiology, (2010) (PubMed).
: "Protective effect of aminoguanidine against nephrotoxicity induced by cisplatin in normal rats." in: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, Vol. 132, Issue 2, pp. 123-8, (2002) (PubMed).
: "Human C-peptide acutely lowers glomerular hyperfiltration and proteinuria in diabetic rats: a dose-response study." in: Naunyn-Schmiedeberg's archives of pharmacology, Vol. 365, Issue 1, pp. 67-73, (2002) (PubMed).
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: "Tubulointerstitial De Novo Expression of the α8 Integrin Chain in a Rodent Model of Renal Fibrosis - A Potential Target for Anti-Fibrotic Therapy?" in: PLoS ONE, Vol. 7, Issue 11, pp. e48362, (2012) (PubMed).
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- 抗原 See all Albumin (ALB) ELISA试剂盒
- Albumin (ALB)
- 别名
- Albumin (ALB 产品)
- 别名
- PRO0883 ELISA Kit, PRO0903 ELISA Kit, PRO1341 ELISA Kit, ALB ELISA Kit, CSA ELISA Kit, Alb-1 ELISA Kit, Alb1 ELISA Kit, Albza ELISA Kit, LOC100136344 ELISA Kit, alb-a ELISA Kit, alb-b ELISA Kit, albumin ELISA Kit, serum albumin 1 ELISA Kit, albumin S homeolog ELISA Kit, ALB ELISA Kit, Alb ELISA Kit, LOC100136344 ELISA Kit, alb.S ELISA Kit
- 背景
- A2M is a main part of the globulin fraction of serum. The normal serum concentration is about 20-60 µg/ml.
- 途径
- Lipid Metabolism
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