Albumin ELISA 试剂盒
(6 references)Quick Overview for Albumin ELISA 试剂盒 (ABIN454012)
抗原
See all Albumin (ALB) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
-
-
最低检测浓度
- 50 ng/mL
-
原理
- The Alpha2-macroglobulin (rat)-ELISA is designed for the quantitative determination of Alpha2-macroglobulin (A2M) in rat body fluids and cell culture supernatants.
-
Analytical Method
- Quantitative
-
产品特性
- The determination of A2M is carried out as competitive ELISA.
-
-
Albumin (ALB) ELISA Kit
ALB 适用: 人 Colorimetric Competition ELISA 246.9 ng/mL - 20000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Albumin (ALB) ELISA KitALB 适用: 小鼠 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 大鼠 Colorimetric Competition ELISA 123.5 ng/mL - 10000 ng/mL Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 大鼠 Colorimetric Sandwich ELISA 3.13 μg/mL - 200 μg/mL Cell Culture Supernatant, Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 大鼠 Colorimetric Sandwich ELISA 7.813 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate
Albumin (ALB) ELISA KitHigh Sensitivity ALB 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 犬 Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
Albumin (ALB) ELISA KitALB 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Urine
Albumin (ALB) ELISA KitALB 适用: 兔 Colorimetric Competition ELISA 617.3 ng/mL - 50000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
-
-
板类型
- Pre-coated
-
实验流程
- A2M has been pre-coated onto a microplate. During incubation the binding of an anti-A2M(rat)-antibody to the wells is inhibited by A2M from samples/standards. The immobilized antibody is detected using a HRP-labelled secondary antibody. After washing away any unbound antibody, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is reverse proportional to the A2M concentration.
-
实验流程
-
Incubation of samples/standards with antibody conjugate: 50 µL + 50 µL/well 60 min, room temperature with shaking
Wash Incubation of detection antibody (HRP-labeled): 100 µL/well 60 min with shaking
Wash Substrate incubation: 100 µL/well 20 min, room temperature
Add stop solution: 100 µL/well Read at 450 nm -
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
-
-
: "Tubulointerstitial De Novo Expression of the α8 Integrin Chain in a Rodent Model of Renal Fibrosis - A Potential Target for Anti-Fibrotic Therapy?" in: PLoS ONE, Vol. 7, Issue 11, pp. e48362, (2012) (PubMed).
: "Angiotensin II Type 1 Receptor Antibodies and Increased Angiotensin II Sensitivity in Pregnant Rats." in: Hypertension, Vol. 58, Issue 1, pp. 77-84, (2011) (PubMed).
: "Lipid phosphatase SHIP2 downregulates insulin signalling in podocytes." in: Molecular and cellular endocrinology, Vol. 328, Issue 1-2, pp. 70-9, (2010) (PubMed).
: "Lack of {alpha}8 integrin aggravates podocyte injury in experimental diabetic nephropathy." in: American journal of physiology. Renal physiology, (2010) (PubMed).
: "Protective effect of aminoguanidine against nephrotoxicity induced by cisplatin in normal rats." in: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, Vol. 132, Issue 2, pp. 123-8, (2002) (PubMed).
: "Human C-peptide acutely lowers glomerular hyperfiltration and proteinuria in diabetic rats: a dose-response study." in: Naunyn-Schmiedeberg's archives of pharmacology, Vol. 365, Issue 1, pp. 67-73, (2002) (PubMed).
-
: "Tubulointerstitial De Novo Expression of the α8 Integrin Chain in a Rodent Model of Renal Fibrosis - A Potential Target for Anti-Fibrotic Therapy?" in: PLoS ONE, Vol. 7, Issue 11, pp. e48362, (2012) (PubMed).
-
- Albumin (ALB)
-
别名
- Albumin
-
背景
- A2M is a main part of the globulin fraction of serum. The normal serum concentration is about 20-60 µg/ml.
-
途径
- Lipid Metabolism
抗原 See all Albumin (ALB) ELISA试剂盒
-
