Myeloperoxidase ELISA 试剂盒
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Quick Overview for Myeloperoxidase ELISA 试剂盒 (ABIN411400)
抗原
See all Myeloperoxidase (MPO) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 312 pg/mL
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原理
- Sandwich Human MPO/Myeloperoxidase ELISA Kit to quantitate Human MPO in cell culture supernatants, cell lysate, serum, plasma (heparin, EDTA) , saliva and urine.
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: A49-S745Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Myeloperoxidase (MPO) ELISA Kit
MPO 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: 大鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: Pig Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: 人 Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: Cow Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: 大鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: 犬 Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: Pig Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: Cow Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 7.4%, 6.8%, 4.2%
Inter-Assay Precision (CV%): 8.2%, 7.9%, 5.5% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "Bradykinin inhibits oxidative stress-induced senescence of endothelial progenitor cells through the B2R/AKT/RB and B2R/EGFR/RB signal pathways." in: Oncotarget, Vol. 6, Issue 28, pp. 24675-89, (2016) (PubMed).
: "Neutrophil serine proteases and their endogenous inhibitors in coronary artery ectasia patients." in: Anatolian journal of cardiology, Vol. 16, Issue 1, pp. 23-8, (2016) (PubMed).
: "The Burkholderia cenocepacia OmpA-like protein BCAL2958: identification, characterization, and detection of anti-BCAL2958 antibodies in serum from B. cepacia complex-infected Cystic Fibrosis patients." in: AMB Express, Vol. 6, Issue 1, pp. 41, (2016) (PubMed).
: "Banxia xiexin decoction protects against dextran sulfate sodium-induced chronic ulcerative colitis in mice." in: Journal of ethnopharmacology, Vol. 166, pp. 149-56, (2015) (PubMed).
: "Biochanin A protects against focal cerebral ischemia/reperfusion in rats via inhibition of p38-mediated inflammatory responses." in: Journal of the neurological sciences, Vol. 348, Issue 1-2, pp. 121-5, (2015) (PubMed).
: "Edaravone rescues the lung by inhibiting lipid peroxidation and pro-inflammatory cytokines in a rat model." in: Chinese medical journal, Vol. 127, Issue 5, pp. 968-70, (2014) (PubMed).
: "Effects of polysaccharides of the Euphoria Longan (Lour.) Steud on focal cerebral ischemia/reperfusion injury and its underlying mechanism." in: Brain injury, Vol. 25, Issue 3, pp. 292-9, (2011) (PubMed).
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- Myeloperoxidase (MPO)
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别名
- MPO
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背景
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Background: Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function. The standard product used in this kit is the product of gene recombination, consisting of 697 (A49-S745) amino acids with the molecular mass of 80KDa.
Gene Full Name: myeloperoxidase
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基因ID
- 4353
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UniProt
- P05164
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途径
- Chromatin Binding
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