Endoglin ELISA 试剂盒
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- 抗原 See all Endoglin (ENG) ELISA试剂盒
- Endoglin (ENG)
- 抗原表位
- AA 26-586
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD105
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: CHO
Immunogen sequence: E26-G586 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <15pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: CHO
Immunogen sequence: E26-G586 - Featured
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Endoglin (ENG) ELISA Kit
ENG 适用: 人 Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum, Tissue Homogenate
Endoglin (ENG) ELISA KitENG 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endoglin (ENG) ELISA KitENG 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Endoglin (ENG) ELISA KitENG 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Endoglin (ENG) ELISA KitENG 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Endoglin (ENG) ELISA KitENG 适用: 犬 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endoglin (ENG) ELISA KitENG 适用: 小鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Cell Lysate, Tissue Homogenate
Endoglin (ENG) ELISA KitENG 适用: 小鼠 Colorimetric Sandwich ELISA 16-4.000 pg/mL Cell Culture Supernatant, Plasma, Serum
Endoglin (ENG) ELISA KitENG 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Endoglin is restricted to endothelial cells in all tissues except bone marrow.
- 板类型
- Pre-coated
- 实验流程
- human CD105 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD105 has been precoated onto 96-well plates. Standards(CHO, E26-G586) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD105 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD105 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CD105 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD105 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 0.62, Standard deviation: 0.022, CV(%): 3.5
- Sample 2: n=16, Mean(ng/ml): 1.67, Standard deviation: 0.078, CV(%): 4.7
- Sample 3: n=16, Mean(ng/ml): 3.59, Standard deviation: 0.151, CV(%): 4.2,
- Sample 1: n=24, Mean(ng/ml): 0.71, Standard deviation: 0.051, CV(%): 7.2
- Sample 2: n=24, Mean(ng/ml): 1.78, Standard deviation: 0.126, CV(%): 7.1
- Sample 3: n=24, Mean(ng/ml): 3.46, Standard deviation: 0.266, CV(%): 7.7
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all Endoglin (ENG) ELISA试剂盒
- Endoglin (ENG)
- 别名
- ENG (ENG 产品)
- 别名
- ENG ELISA Kit, MGC137842 ELISA Kit, DKFZp469D0419 ELISA Kit, END ELISA Kit, HHT1 ELISA Kit, ORW1 ELISA Kit, AI528660 ELISA Kit, AI662476 ELISA Kit, CD105 ELISA Kit, S-endoglin ELISA Kit, endoglin ELISA Kit, ENG ELISA Kit, Eng ELISA Kit
- 背景
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Protein Function: Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF- beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGF-beta1 signaling through SMAD3. .
Background: CD105, also called Endoglin, is a homodimeric membrane glycoprotein primarily associated with human vascular endothelium. It is also found on bone marrow proerythroblasts, activated monocytes, and lymphoblasts in childhood leukemia. Endoglin is a component of the transforming growth factor-beta(TGFB) receptor complex and binds TGFB1 with high affinity.1 CD105 gene is mapped to 9q34.1. The coding region of the gene contains 14 exons.2 The protein consists of a homodimer of 180 kDA with disulfide links. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. Furthermore, it also has a role in the balance of ALK1 and ALK5 signaling to regulate endothelial cell proliferation.3 Moreover, the elevated expression of endoglin in the surgically excised CNVMs suggested a persisting postmitotic activation in an advanced stage of neovascular tissue.4 The standard product used in this kit is extracellular part of recombinant human CD105, from E26 to G586. As a result of glycosylation, the molecular mass is 75-85KDa.
Synonyms: Endoglin,CD105,ENG,END,
Full Gene Name: Endoglin
Cellular Localisation: Membrane, Single-pass type I membrane protein. - 基因ID
- 2022
- UniProt
- P17813
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