Thrombospondin 2 ELISA 试剂盒
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Quick Overview for Thrombospondin 2 ELISA 试剂盒 (ABIN411388)
抗原
See all Thrombospondin 2 (THBS2) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 625 pg/mL
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原理
- Sandwich Human TSP2 ELISA Kit to quantitate Human THBS2 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: G19-I1172Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Thrombospondin 2 (THBS2) ELISA Kit
THBS2 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum
Thrombospondin 2 (THBS2) ELISA KitTHBS2 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Thrombospondin 2 (THBS2) ELISA KitTHBS2 适用: 人 Colorimetric Sandwich ELISA 0.47 ng/mL - 30 ng/mL Plasma
Thrombospondin 2 (THBS2) ELISA KitTHBS2 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Tissue Homogenate
Thrombospondin 2 (THBS2) ELISA KitTHBS2 适用: 小鼠 Colorimetric Sandwich ELISA 0.5 ng/mL - 32 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Thrombospondin 2 (THBS2) ELISA KitTHBS2 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum
Thrombospondin 2 (THBS2) ELISA KitTHBS2 适用: 小鼠 Colorimetric Sandwich ELISA 0.16-40 ng/mL Cell Culture Supernatant, Plasma, Serum
Thrombospondin 2 (THBS2) ELISA KitTHBS2 适用: 人 Colorimetric Sandwich ELISA 157-10000 pg/mL Cell Lysate, Plasma, Serum
Thrombospondin 2 (THBS2) ELISA KitTHBS2 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Tissue Homogenate
Thrombospondin 2 (THBS2) ELISA KitTHBS2 适用: 人 Colorimetric Sandwich ELISA 0.47 ng/mL - 30 ng/mL Plasma, Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 7.5%, 4.6%, 6.8%
Inter-Assay Precision (CV%): 8%, 6.5%, 7.7% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- Thrombospondin 2 (THBS2)
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别名
- THBS2
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背景
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Background: Thrombospondin-2 (TSP-2) is a protein that in humans is encoded by the THBS2 gene.And this gene is mapped to 6q27. The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. TSP-2 has been shown to interact with MMP2.
Gene Full Name: thrombospondin 2
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基因ID
- 7058
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UniProt
- P35442
抗原 See all Thrombospondin 2 (THBS2) ELISA试剂盒
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