Thrombospondin 2 ELISA 试剂盒
Quick Overview for Thrombospondin 2 ELISA 试剂盒 (ABIN411388)
抗原
See all Thrombospondin 2 (THBS2) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TSP2
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
- Expression system for standard: NSO,G19-I1172
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: G19-I1172
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Thrombospondin 2 (THBS2) ELISA Kit
THBS2 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum
Thrombospondin 2 (THBS2) ELISA KitTHBS2 适用: 人 Colorimetric Sandwich ELISA 0.47 ng/mL - 30 ng/mL Plasma
Thrombospondin 2 (THBS2) ELISA KitTHBS2 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Thrombospondin 2 (THBS2) ELISA KitTHBS2 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Thrombospondin 2 (THBS2) ELISA KitTHBS2 适用: 小鼠 Colorimetric Sandwich ELISA 0.5 ng/mL - 32 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Thrombospondin 2 (THBS2) ELISA KitTHBS2 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Tissue Homogenate
Thrombospondin 2 (THBS2) ELISA KitTHBS2 适用: 小鼠 Colorimetric Sandwich ELISA 0.16-40 ng/mL Cell Culture Supernatant, Plasma, Serum
Thrombospondin 2 (THBS2) ELISA KitTHBS2 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the thrombospondin family.
Tissue Specificity: High expression in invertebral disk tissue. .
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板类型
- Pre-coated
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实验流程
- human TSP2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TSP2 has been precoated onto 96-well plates. Standards (NSO,G19-I1172) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TSP2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TSP2 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TSP2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA) or human milk to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TSP2 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 0.56, Standard deviation: 0.04, CV(%): 7.1
- Sample 2: n=16, Mean(ng/ml): 1.29, Standard deviation: 0.08, CV(%): 6.2
- Sample 3: n=16, Mean(ng/ml): 5.84, Standard deviation: 0.32, CV(%): 5.5,
- Sample 1: n=24, Mean(ng/ml): 0.62, Standard deviation: 0.05, CV(%): 8.1
- Sample 2: n=24, Mean(ng/ml): 1.36, Standard deviation: 0.1, CV(%): 7.4
- Sample 3: n=24, Mean(ng/ml): 6.47, Standard deviation: 0.42, CV(%): 6.5
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- Thrombospondin 2 (THBS2)
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别名
- THBS2
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背景
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Protein Function: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties. .
Background: Thrombospondin-2(TSP-2) is a protein that in humans is encoded by the THBS2 gene.And this gene is mapped to 6q27. The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. TSP-2 has been shown to interact with MMP2.
Synonyms: Thrombospondin-2,THBS2,TSP2,
Full Gene Name: Thrombospondin-2
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基因ID
- 7058
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UniProt
- P35442
抗原 See all Thrombospondin 2 (THBS2) ELISA试剂盒
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